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首页> 外文期刊>Applied Microbiology >Comparative Genomic Analysis and Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylene (BTEX) Degradation Pathways of Pseudoxanthomonas spadix BD-a59
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Comparative Genomic Analysis and Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylene (BTEX) Degradation Pathways of Pseudoxanthomonas spadix BD-a59

机译：比较基因组分析和假黄单胞菌BD-a59的苯，甲苯，乙苯和邻，间和对二甲苯（BTEX）降解途径

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Pseudoxanthomonas spadix BD-a59, isolated from gasoline-contaminated soil, has the ability to degrade all six BTEX (benzene, toluene, ethylbenzene, and o -, m -, and p -xylene) compounds. The genomic features of strain BD-a59 were analyzed bioinformatically and compared with those of another fully sequenced Pseudoxanthomonas strain, P. suwonensis 11-1, which was isolated from cotton waste compost. The genome of strain BD-a59 differed from that of strain 11-1 in many characteristics, including the number of rRNA operons, dioxygenases, monooxygenases, genomic islands (GIs), and heavy metal resistance genes. A high abundance of phage integrases and GIs and the patterns in several other genetic measures (e.g., GC content, GC skew, Karlin signature, and clustered regularly interspaced short palindromic repeat [CRISPR] gene homology) indicated that strain BD-a59's genomic architecture may have been altered through horizontal gene transfers (HGT), phage attack, and genetic reshuffling during its evolutionary history. The genes for benzene/toluene, ethylbenzene, and xylene degradations were encoded on GI-9, -13, and -21, respectively, which suggests that they may have been acquired by HGT. We used bioinformatics to predict the biodegradation pathways of the six BTEX compounds, and these pathways were proved experimentally through the analysis of the intermediates of each BTEX compound using a gas chromatograph and mass spectrometry (GC-MS). The elevated abundances of dioxygenases, monooxygenases, and rRNA operons in strain BD-a59 (relative to strain 11-1), as well as other genomic characteristics, likely confer traits that enhance ecological fitness by enabling strain BD-a59 to degrade hydrocarbons in the soil environment.
机译：从汽油污染的土壤中分离出来的假黄单胞菌BD-a59具有降解所有六种BTEX（苯，甲苯，乙苯和邻，间和对二甲苯）化合物的能力。对BD-a59菌株的基因组学特征进行了生物信息学分析，并与从棉花废料堆肥中分离的另一种全序列假单胞菌P. suwonensis 11-1进行了比较。 BD-a59菌株的基因组在许多特征上与11-1菌株不同，包括rRNA操纵子，双加氧酶，单加氧酶，基因组岛（GIs）和重金属抗性基因的数量。大量的噬菌体整合和胃肠道以及其他几种遗传学检测方法（例如，GC含量，GC偏斜，Karlin签名以及成簇的规则间隔的短回文重复[CRISPR]基因同源性）的模式表明，菌株BD-a59的基因组结构可能在其进化史中，通过水平基因转移（HGT），噬菌体攻击和基因改组已经改变了它们。苯/甲苯，乙苯和二甲苯降解的基因分别在GI-9，-13和-21上编码，这表明它们可能已被HGT获得。我们使用生物信息学预测了六种BTEX化合物的生物降解途径，并通过使用气相色谱仪和质谱（GC-MS）分析每种BTEX化合物的中间体，对这些途径进行了实验验证。 BD-a59菌株（相对于11-1菌株）中双加氧酶，单加氧酶和rRNA操纵子的丰度升高，以及其他基因组特征，可能通过使BD-a59菌株降解碳氢化合物中的碳氢化合物来赋予增强生态适应性的性状。土壤环境。

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