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首页> 外文期刊>Applied Microbiology >Aspergillus Collagen-Like Genes (acl): Identification, Sequence Polymorphism, and Assessment for PCR-Based Pathogen Detection
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Aspergillus Collagen-Like Genes (acl): Identification, Sequence Polymorphism, and Assessment for PCR-Based Pathogen Detection

机译：曲霉样胶原蛋白基因（acl）：鉴定，序列多态性和基于PCR的病原体检测评估。

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The genus Aspergillus is a burden to public health due to its ubiquitous presence in the environment, its production of allergens, and wide demographic susceptibility among cystic fibrosis, asthmatic, and immunosuppressed patients. Current methods of detection of Aspergillus colonization and infection rely on lengthy morphological characterization or nonstandardized serological assays that are restricted to identifying a fungal etiology. Collagen-like genes have been shown to exhibit species-specific conservation across the noncollagenous regions as well as strain-specific polymorphism in the collagen-like regions. Here we assess the conserved region of the Aspergillus collagen-like ( acl ) genes and explore the application of PCR amplicon size-based discrimination among the five most common etiologic species of the Aspergillus genus, including Aspergillus fumigatus , A. flavus , A. nidulans , A. niger , and A. terreus . Genetic polymorphism and phylogenetic analysis of the aclF1 gene were additionally examined among the available strains. Furthermore, the applicability of the PCR-based assay to identification of these five species in cultures derived from sputum and bronchoalveolar fluid from 19 clinical samples was explored. Application of capillary electrophoresis on nanogels was additionally demonstrated to improve the discrimination between Aspergillus species. Overall, this study demonstrated that Aspergillus acl genes could be used as PCR targets to discriminate between clinically relevant Aspergillus species. Future studies aim to utilize the detection of Aspergillus acl genes in PCR and microfluidic applications to determine the sensitivity and specificity for the identification of Aspergillus colonization and invasive aspergillosis in immunocompromised subjects.
机译：由于曲霉菌在环境中的普遍存在，其变应原的产生以及在囊性纤维化，哮喘和免疫抑制患者中广泛的人口易感性，因此曲霉菌是公共卫生的负担。目前检测曲霉菌定殖和感染的方法依赖于冗长的形态学表征或仅限于鉴定真菌病因的非标准化血清学测定。胶原蛋白样基因已显示出跨非胶原区域的物种特异性保守性以及胶原蛋白样区域中的菌株特异性多态性。在这里，我们评估了曲霉胶原样（acl）基因的保守区域，并探讨了基于PCR扩增子大小判别的曲霉属五个最常见病原学物种的应用，包括烟曲霉，黄曲霉，构巢曲霉。 ，A. niger和A. terreus。在可用菌株中，还检查了aclF1基因的遗传多态性和系统发育分析。此外，探索了基于PCR的测定方法在鉴定源自19个临床样本的痰液和支气管肺泡液的培养物中这五个物种的适用性。另外证明了在纳米凝胶上应用毛细管电泳可改善曲霉菌种之间的区别。总的来说，这项研究表明曲霉acl基因可以用作PCR靶标，以区分临床上相关的曲霉菌种。未来的研究旨在利用PCR和微流控应用中的曲霉acl基因检测来确定在免疫受损受试者中鉴定曲霉菌定植和侵袭性曲霉病的敏感性和特异性。

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