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首页> 外文期刊>Applied Microbiology >Identification of CtpL as a Chromosomally Encoded Chemoreceptor for 4-Chloroaniline and Catechol in Pseudomonas aeruginosa PAO1
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Identification of CtpL as a Chromosomally Encoded Chemoreceptor for 4-Chloroaniline and Catechol in Pseudomonas aeruginosa PAO1

机译：CtpL作为铜绿假单胞菌PAO1中4-氯苯胺和邻苯二酚的染色体编码化学受体的鉴定

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Bacterial chemotaxis influences the ability of bacteria to survive and thrive in most environments, including polluted ones. Despite numerous reports of the phenotypic characterization of chemotactic bacteria, only a few molecular details of chemoreceptors for aromatic pollutants have been described. In this study, the molecular basis of chemotaxis toward an environmentally toxic chlorinated aromatic pollutant, 4-chloroaniline (4CA), was evaluated. Among the three Pseudomonas spp. tested, Pseudomonas aeruginosa PAO1 exhibited positive chemotaxis both to the nonmetabolizable 4CA, where 4-chloroacetanilide was formed as a dead-end transformation product, and to the metabolizable catechol. Molecular analysis of all 26 mutants with a disrupted methyl-accepting chemotaxis gene revealed that CtpL, a chromosomally encoded chemoreceptor, was responsible for the positive chemotactic response toward 4CA. Since CtpL has previously been described to be a major chemoreceptor for inorganic phosphate at low concentrations in PAO1, this report describes a fortuitous ability of CtpL to function toward aromatic pollutants. In addition, its regulation not only was dependent on the presence of the chemoattractant inducer but also was regulated by conditions of phosphate starvation. These results expand the range of known chemotactic transducers and their function in the environmental bacterium PAO1.
机译：细菌趋化性会影响细菌在大多数环境（包括污染环境）中存活和繁殖的能力。尽管有许多关于趋化细菌的表型表征的报道，但是仅描述了芳香污染物的化学感受器的一些分子细节。在这项研究中，对趋向于对环境有毒的氯化芳族污染物4-氯苯胺（4CA）进行趋化作用的分子基础进行了评估。在三个假单胞菌属中。在测试中，铜绿假单胞菌PAO1对不可代谢的4CA和可代谢的邻苯二酚均表现出正趋化性，其中不可代谢的4CA形成4-氯乙酰苯胺作为末端转化产物。对具有破坏性的甲基接受趋化性基因的所有26个突变体进行的分子分析表明，染色体编码的化学感受器CtpL负责对4CA的阳性趋化反应。由于以前已经描述过CtpL是低浓度的PAO1中无机磷酸盐的主要化学感受器，因此本报告描述了CtpL对芳香族污染物起作用的偶然能力。另外，其调节不仅取决于化学引诱剂的存在，而且还受磷酸盐饥饿条件的调节。这些结果扩大了已知趋化换能器的范围及其在环境细菌PAO1中的功能。

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