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首页> 外文期刊>Applied Microbiology >Phylogenetic Differences in Attached and Free-Living Bacterial Communities in a Temperate Coastal Lagoon during Summer, Revealed via High-Throughput 16S rRNA Gene Sequencing
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Phylogenetic Differences in Attached and Free-Living Bacterial Communities in a Temperate Coastal Lagoon during Summer, Revealed via High-Throughput 16S rRNA Gene Sequencing

机译：通过高通量16S rRNA基因测序揭示了温带沿海泻湖夏季附着和自由生活的细菌群落的系统发育差异。

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Most of what is known about coastal free-living and attached bacterial diversity is based on open coasts, with high particulate and nutrient riverine supply, terrestrial runoffs, and anthropogenic activities. The Magdalen Islands in the Gulf of St. Lawrence (Canada) are dominated by shallow lagoons with small, relatively pristine catchments and no freshwater input apart from rain. Such conditions provided an opportunity to investigate coastal free-living and attached marine bacterial diversity in the absence of confounding effects of steep freshwater gradients. We found significant differences between the two communities and marked temporal patterns in both. Taxonomic richness and diversity were greater in the attached than in the free-living community, increasing over summer, especially within the least abundant bacterial phyla. The highest number of reads fell within the SAR 11 clade ( Pelagibacter , Alphaproteobacteria ), which dominated free-living communities. The attached communities had deeper phylum-level diversity than the free-living fraction. Distance-based redundancy analysis indicated that the particulate organic matter (POM) concentration was the main variable separating early and late summer samples with salinity and temperature changes also significantly correlated to bacterial community structure. Our approach using high-throughput sequencing detected differences in free-living versus attached bacteria in the absence of riverine input, in keeping with the concept that marine attached communities are distinct from cooccurring free-living taxa. This diversity likely reflects the diverse microhabitats of available particles, implying that the total bacterial diversity in coastal systems is linked to particle supply and variability, with implications for understanding microbial biodiversity in marine systems.
机译：关于沿海自由生活和附着的细菌多样性的大多数已知知识都是基于开阔海岸，具有大量的颗粒和养分的河流供应，陆地径流和人为活动。圣劳伦斯湾（加拿大）的马格达林群岛被浅泻湖所控制，这些泻湖具有相对较小的原始集水区，除雨水外没有淡水输入。在没有陡峭的淡水梯度的混杂影响的情况下，这种条件为调查沿海自由生活和附属的海洋细菌多样性提供了机会。我们发现两个社区之间存在显着差异，并且在两个社区中都有明显的时间格局。依附的分类学丰富度和多样性要大于自由生活的群落，在整个夏季特别是细菌门最不丰富的地方，尤其如此。读取次数最多的是属于自由生活社区的SAR 11进化枝（Pelagibacter，Alphaproteobacteria）。附属的社区比自由生活的部分具有更深的门类多样性。基于距离的冗余分析表明，颗粒有机物（POM）浓度是分离初夏和夏末样品的主要变量，盐度和温度变化也与细菌群落结构显着相关。我们的方法使用高通量测序，在没有河流输入的情况下，检测了自由生活细菌和附着细菌的差异，这与海洋附着社区不同于同时发生的自由生活类群的概念保持一致。这种多样性可能反映了可用颗粒物的不同微生物栖息地，这意味着沿海系统中的总细菌多样性与颗粒物的供应和可变性相关，对理解海洋系统中的微生物多样性具有影响。

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