Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension

Stanley C. Nagle

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Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension

机译：悬浮动物细胞生长的热稳定化学定义培养基

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Procedures are described for preparation of a chemically defined medium, capable of being autoclaved, for the cultivation of animal cells in suspension. Yields of 34.1 × 105, 33.8 × 105, and 47.1 × 105 cells per ml were recorded for cat kidney, HeLa, and mouse fibroblast (L) cells after 6, 10, and 10 days of incubation, respectively.

机译：描述了制备能够高压灭菌的化学成分确定的培养基的方法，该培养基用于悬浮培养动物细胞。孵育6天，10天和10天后，猫肾，HeLa和小鼠成纤维细胞（L）细胞的每毫升产量分别为34.1×105、33.8×105和47.1×105个细胞。

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《Applied Microbiology》 |1968年第1期|共3页
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Stanley C. Nagle;
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中图分类微生物学;
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机译：四氢叶酸增加化学定义的培养基中缺乏二氢叶酸还原酶的中国仓鼠卵巢DG44细胞的悬浮生长。
3. Growth and Metabolism of L Cells in a Chemically Defined Medium in a Controlled Environment Culture System I. Effects of O2 Tension on L-Cell Cultures [J] . G. W. Taylor, J. P. Kondig, S. C. Nagle, Applied Microbiology . 1971,第5期

机译：受控环境培养系统中化学成分确定的培养基中L细胞的生长和代谢I. O2张力对L细胞培养的影响
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机译：用于悬浮MDCK细胞的流感疫苗生产的化学定义，无动物成分的培养基的开发
5. Comparative Efficacy of Bone Marrow-Derived Mesenchymal Stem Cell Growth and Differentiation Between Animal Component-Free and Fetal Bovine Serum Supplemented Medium [D] . Diaz, Stephanie. 2019

机译：骨髓衍生间充质干细胞生长和分化动物组分和胎儿牛血清补充培养基的比较疗效
6. Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension [O] . Stanley C. Nagle Jr. 1968

机译：悬浮动物细胞生长的热稳定化学定义培养基
7. Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension [O] . Nagle Stanley C. 1968

机译：悬浮动物细胞生长的热稳定化学定义培养基

Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension

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