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Nitrite-induced Germination of Putrefactive Anaerobe 3679h Spores

机译：亚硝酸盐诱导的腐烂厌氧菌3679h孢子萌发

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摘要

Sodium nitrite alone has been shown to stimulate germination of PA 3679h spores. The process was accelerated by using increased concentrations of sodium nitrite, a low pH, and a high temperature of incubation. At low concentrations of nitrite (0.01 to 0.2%), the delay of 36 to 48 hr occurred before germination commenced at 37 C. However, with 3.45% nitrite at 45 C and pH 6.0, most of the spores germinated within 1 hr. At pH 7.0, the germination rate decreased markedly, and at pH 8.0 it was nil. The greatest acceleration in germination rate occurred near 60 C. Hydroxylamine was completely inhibitory to nitrite-induced germination. Sodium nitrite, in turn, inhibited germination by l-alanine, the degree of inhibition being influenced by nitrite concentration and pH.
机译：已显示仅亚硝酸钠可刺激PA 3679h孢子萌发。通过增加浓度的亚硝酸钠，较低的pH和较高的孵育温度，可以加快该过程。在低浓度的亚硝酸盐（0.01至0.2 ％）下，在37°C开始萌发之前发生了36至48小时的延迟。但是，在45°C和pH 6.0的亚硝酸盐为3.45％的情况下，大多数孢子在1小时内萌发了。在pH 7.0时，发芽率显着降低，在pH 8.0时，发芽率为零。发芽速率的最大加速发生在60°C附近。羟胺完全抑制了亚硝酸盐诱导的发芽。反过来，亚硝酸钠抑制1-丙氨酸的发芽，抑制的程度受亚硝酸盐浓度和pH的影响。

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