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首页> 外文期刊>Applied Microbiology >Radiation Treatment of Foods II. Public Health Significance of Irradiation-Recycled Salmonella
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Radiation Treatment of Foods II. Public Health Significance of Irradiation-Recycled Salmonella

机译：食品的放射治疗II。辐照回收沙门氏菌的公共卫生意义

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Salmonellae resistant to gamma irradiation were developed by repeated irradiation and subculturing in a nutrient broth-yeast extract medium. Few differences were noted in the biochemical characteristics of parent and resistant cultures; however, microculture studies revealed variations in morphology and in cell division patterns. A considerable decrease in pathogenicity for day-old chicks was apparent with resistant cultures, but their phenol-water extracts were as toxic as parent material for 10-day chick embryos. Five serial chick passages did not reverse the reduced pathogenicity or aberrant morphology of a resistant Salmonella typhimurium culture. Results of phage typing of both parent and serially irradiated S. typhimurium were inconclusive, whereas the O-1 genus-specific phage lysed all parent serotypes tested but only one of the serially irradiated cultures. Agglutination of parent S. typhimurium cells with their homologous rabbit antiserum was unaffected by prior absorption with resistant strains. The results indicate that radiation recycling altered Salmonella into strains of lesser public health significance.
机译：通过反复照射并在营养肉汤酵母提取物培养基中继代培养出对伽玛射线具有抗性的沙门氏菌。亲本和抗性培养物的生化特性几乎没有差异。然而，微培养研究揭示了形态和细胞分裂模式的变化。在抗性培养中，日龄雏鸡的致病性明显降低，但是它们的酚水提取物的毒性与10天雏鸡的母体材料一样。五个连续的小鸡传代并没有逆转鼠伤寒沙门氏菌培养物的致病性降低或异常形态。亲本和连续照射的鼠伤寒沙门氏菌噬菌体分型的结果尚无定论，而O-1属特异性噬菌体溶解了所有受试的父母血清型，但只有一种连续照射的培养物。亲本鼠伤寒沙门氏菌细胞与其同源兔抗血清的凝集不受事先被抗性菌株吸收的影响。结果表明，辐射回收将沙门氏菌变成了对公共卫生影响较小的菌株。

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