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首页> 外文期刊>Applied Microbiology >Growth Responses of Escherichia coli to the Surfactant Dodecyl Benzene Sulfonate
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Growth Responses of Escherichia coli to the Surfactant Dodecyl Benzene Sulfonate

机译：大肠杆菌对表面活性剂十二烷基苯磺酸盐的生长响应

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When strains of Escherichia coli are grown in broth cultures containing the anionic surfactant sodium dodecyl benzene sulfonate (NaDDBS), they exhibit unique growth responses. After 20 to 24 hr of incubation, they become slimy and viscous, and an addition of ethanol to the supernatant liquid yields a distinctive white, fibrous precipitate. The production of this material was shown to be dependent on the presence of NaDDBS in the medium. This precipitate from E. coli ATCC 11303 was found to contain 41 to 53% protein, 10 to 11% deoxyribonucleic acid, 6.8 to 7.4% ribonucleic acid, 15 to 25% carbohydrate, and 9% lipid. It is distinctive from naturally occurring E. coli slimes in several respects. Our data suggest that its formation is the primary result of the leakage of intracellular components into the medium. However, the rate of cell proliferation indicates a partial but not complete or lethal lysis. A limited utilization of NaDDBS as a carbon source was also shown.
机译：当大肠杆菌菌株在含有阴离子表面活性剂十二烷基苯磺酸钠（NaDDBS）的肉汤培养物中生长时，它们表现出独特的生长反应。孵育20至24小时后，它们变得粘稠粘稠，向上清液中添加乙醇会产生明显的白色纤维状沉淀。该物质的产生显示出取决于培养基中NaDDBS的存在。发现来自大肠杆菌ATCC 11303的沉淀物包含41至53％的蛋白质，10至11％的脱氧核糖核酸，6.8至7.4％的核糖核酸，15至25％的碳水化合物和9％的脂质。它在几个方面与天然的大肠杆菌粘液不同。我们的数据表明，它的形成是细胞内组分泄漏到培养基中的主要结果。然而，细胞增殖的速率表明部分但不是完全或致命的裂解。还显示了NaDDBS作为碳源的利用有限。

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