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首页> 外文期刊>Applied Microbiology >Mycoplasma: Growth Precipitation as a Serodiagnostic Method
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Mycoplasma: Growth Precipitation as a Serodiagnostic Method

机译：支原体：生长沉淀作为一种血清学诊断方法

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摘要

A serological test is described which is designated the growth precipitation test and is based on the release of diffusible compounds from mycoplasma strains. During growth of mycoplasmas on solid substrates, precipitation lines are formed with antibodies from homologous antisera independent of heat-labile serum factors. The application of the test as a serodiagnostic tool was investigated by performing a complete serological cross-test with 10 mycoplasma strains. The test was compared with the growth inhibition test, the complement fixation test, and the indirect hemagglutination test. It is demonstrated that the growth precipitation test has a good degree of specificity. The species and subspecies used here showed cross-reactions between serologically closely related strains, but these heterologous reactions occurred only after longer incubation than the homologous reactions.
机译：描述了一种血清学测试，该测试被称为生长沉淀测试，并且基于从支原体菌株中扩散的化合物的释放。在支原体在固体基质上生长的过程中，沉淀线是由来自同源抗血清的抗体形成的，而与热不稳定的血清因子无关。通过对10种支原体菌株进行完整的血清学交叉测试，研究了该测试作为血清学诊断工具的应用。将该测试与生长抑制测试，补体固定测试和间接血凝测试进行比较。证明了生长沉淀试验具有良好的特异性。此处使用的物种和亚种显示出血清学上密切相关的菌株之间的交叉反应，但是这些异源反应仅在比同源反应孵育更长的时间后发生。

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