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Virus Particles from Conidia of Penicillium Species

机译：青霉分生孢子的病毒颗粒

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Virus particles and their component double-stranded ribonucleic acid (dsRNA) have been isolated from conidia and mycelia of certain Penicillium species. The conidia and mycelia of P. stoloniferum NRRL 5267 contained 75 and 85 μg of dsRNA/g (dry weight), respectively. Of the total dsRNA released from NRRL 5267 conidia, 10% was nonencapsulated. Conidia of P. brevi-compactum NRRL 5260 and P. chrysogenum Q-176 contained 2 and 120 μg of dsRNA/g (dry weight), respectively, whereas mycelium from the two species contained 3 and 95 μg of dsRNA/g (dry weight), respectively. No viruses were isolated from conidia or mycelia of P. stoloniferum NRRL 859. A method is described for disruption of both conidia and mycelia. The technique facilitates the isolation and characterization of fungal viruses and their component dsRNA and also potentiates surveying of fungal isolates for the presence of virus.
机译：从某些青霉菌种的分生孢子和菌丝体中分离出病毒颗粒及其组分双链核糖核酸（dsRNA）。 stoloniferum NRRL 5267的分生孢子和菌丝体分别含有75和85μgdsRNA / g（干重）。从NRRL 5267分生孢子释放的总dsRNA中，有10％未封装。短缩疟原虫NRRL 5260和产黄青霉Q-176的分生孢子分别含有2和120μgdsRNA / g（干重），而来自两个物种的菌丝体含有3和95μgdsRNA / g（干重）。 ）， 分别。没有从腐生假单胞菌NRRL 859的分生孢子或菌丝体中分离出病毒。描述了一种用于破坏分生孢子和菌丝体的方法。该技术有助于真菌病毒及其组分dsRNA的分离和表征，并且还有助于对真菌分离物进行病毒检测。

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