Extraction of Equine Infectious Anemia Immunodiffusion Antigen with the Aid of the Chaotropic Agent, Thiocyanate

L. T. Hart; E. A. Broussard

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Extraction of Equine Infectious Anemia Immunodiffusion Antigen with the Aid of the Chaotropic Agent, Thiocyanate

机译：离液剂硫氰酸盐的帮助下提取马传染性贫血免疫扩散抗原

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Immunodiffusion antigen from spleens of horses infected with equine infectious anemia virus was prepared by methods employing freeze-thaw cycles and thiocyanate treatment. Thiocyanate (0.5 M) permitted the recovery of the greatest amount of antigen. Furthermore, it was most effective for recovery of immunodiffusion antigen from spleens which yielded unsatisfactory concentrations of antigen by the conventional freeze-thaw or water-extraction methods. The reactivity of the antigen did not appear to be affected by this chemical treatment.

机译：通过采用冻融循环和硫氰酸盐处理的方法，制备了被马传染性贫血病毒感染的马的脾脏的免疫扩散抗原。硫氰酸盐（0.5 M）可以回收最大量的抗原。此外，它对于从脾脏中回收免疫扩散抗原是最有效的，而通过常规的冻融或水提取方法，脾脏中产生的抗原浓度不能令人满意。抗原的反应性似乎不受此化学处理的影响。

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《Applied Microbiology》 |1973年第2期|共5页
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L. T. Hart; E. A. Broussard;
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1. Standardization and validation of an agar gel immunodiffusion test for the diagnosis of equine infectious anemia using a recombinant p26 antigen [J] . Alvarez I, Gutierrez G, Vissani A, Veterinary Microbiology . 2007,第3a4期

机译：琼脂凝胶免疫扩散试验用于重组p26抗原诊断马传染性贫血的标准化和验证
2. Comparison of two agar gel immunodiffusion protocols for diagnosing equine infectious anemia [J] . Manabu NEMOTO, Takashi YAMANAKA, Hiroshi BANNAI, The Journal of Veterinary Medical Science . 2018,第8期

机译：两种琼脂凝胶免疫扩散方案诊断马传染性贫血的比较
3. Enzyme-linked immunosorbent assay and agar gel immunodiffusion assay for diagnosis of equine infectious anemia employing p26 protein fused to the maltose-binding protein [J] . Fontes Karin F. L. P., Silva-Junior Luiz C., Nascimento Sergio A., Archives of virology . 2018,第10期

机译：酶联免疫吸附测定和琼脂凝胶免疫分子测定用于诊断马耳他蛋白与麦芽糖结合蛋白融合的血液传染性贫血的诊断
4. Report of the Equine infectious Anemia Subcommittee to the USAHA Committee on Infectious Diseases of Horses [C] . Steven L. Halstead Annual Meeting of the United States Animal Health Association . 2008

机译：马群传染性贫血小组委员会对马鞍山传染病委员会的报告
5. Evidence for the maturation of cellular immune responses in equine infectious anemia virus-infected ponies [D] . Liu, Chong 2013

机译：马传染性贫血病毒感染的小马中细胞免疫反应成熟的证据
6. Extraction of Equine Infectious Anemia Immunodiffusion Antigen with the Aid of the Chaotropic Agent Thiocyanate [O] . L. T. Hart, E. A. Broussard II 1973

机译：离液剂硫氰酸盐的帮助下提取马传染性贫血免疫扩散抗原
7. Extraction of Equine Infectious Anemia Immunodiffusion Antigen with the Aid of the Chaotropic Agent, Thiocyanate [O] . L. T. Hart, E. A. Broussard 1973

机译：借助于络合剂，硫氰酸盐的辅助萃取马传染性贫血免疫分离抗原

Extraction of Equine Infectious Anemia Immunodiffusion Antigen with the Aid of the Chaotropic Agent, Thiocyanate

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