Comparative Sensitivity of Gel-Diffusion and Tube Agglutination Tests for the Detection of Brucella canis Antibodies in Experimentally Infected Dogs

Donald M. Myers; Víctor M. Varela-Díaz; Emilio A. Coltorti

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Comparative Sensitivity of Gel-Diffusion and Tube Agglutination Tests for the Detection of Brucella canis Antibodies in Experimentally Infected Dogs

机译：凝胶扩散和管凝集试验对实验感染狗中布鲁氏菌抗体检测的敏感性比较

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The microslide gel-diffusion and macro-tube agglutination techniques to detect Brucella canis antibodies in dogs were compared. Sera from dogs experimentally infected with B. canis and a random sample of dog sera with unknown histories of exposure to this organism were examined. The results of the gel-diffusion method employing specific rough Brucella saline-extract antigens of B. canis and Brucella ovis were comparable to those obtained by the tube agglutination test. The easily prepared, stable R antigen in the freeze-dried form offers a convenient, simple, and reliable diagnostic method for the serological detection of canine brucellosis by the gel-diffusion test.

机译：比较了检测狗布鲁氏菌抗体的微玻片凝胶扩散法和大管凝集技术。检查了来自实验感染犬双歧杆菌的狗的血清和具有未知暴露于该生物的历史的狗血清的随机样本。使用犬双歧杆菌和猪布鲁氏菌的特定粗布鲁氏菌盐水提取抗原的凝胶扩散方法的结果与通过管凝集试验获得的结果相当。易于制备，稳定的冷冻干燥R抗原为通过凝胶扩散试验进行血清布鲁氏菌病的血清学检测提供了一种方便，简单且可靠的诊断方法。

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《Applied Microbiology》 |1974年第1期|共4页
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Donald M. Myers; Víctor M. Varela-Díaz; Emilio A. Coltorti;
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中图分类微生物学;
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1. Antibodies produced by dogs successfully challenged with live Leptospira spp. did not cross-react to Brucella antigen in a commercial rapid slide agglutination test that detects antibodies to Brucella canis [J] . Krecic Matthew R. Journal of Veterinary Diagnostic Investigation . 2019,第1期

机译：狗产生的抗体成功挑战活瘦身血清SPP。在商业快速载玻片凝集试验中没有对布鲁氏菌抗原交叉反应，检测抗Brucella Canis的抗体
2. Prevalence of Brucella antibodies on a previously acute brucellosis infected population: sensitivity, specificity and predictive values of Rose Bengal and Wright standard tube agglutination tests [J] . Andriopoulos Panagiotis, Kalogerakou Antonia, Rebelou Dimitra, Infection . 2015,第3期

机译：在以前的急性布鲁氏菌病感染人群中布鲁氏菌抗体的流行：玫瑰红和赖特标准管凝集试验的敏感性，特异性和预测值
3. Evaluation of a Rapid Immunochromatographic Dipstick Test for Detection of Antibodies to Trypanosoma cruzi in Dogs Experimentally Infected with Isolates Obtained from Opossums (Didelphis virginiana), Armadillos (Dasypus novemcinctus), and Dogs (Canis familiaris) from the United States [J] . Rosypal Alexa C, Hill Roderick, Lewis Samantha, Journal of Parasitology . 2011,第1期

机译：快速免疫色谱检测试纸的评估，用于检测实验感染来自负鼠（Didelphis virginiana），犰狳（Dasypus novemcinctus）和狗（Canis Friendlyis）的分离株实验感染的狗中的克氏锥虫抗体
4. CLINICALLY SIGNIFICANT DIFFERENCES IN SENSITIVITY LEVELS OF IN-CLINIC RAPID TESTS FOR ANTIBODIES TO ANAPLASMA SPP. IN DOGS [C] . Jiavou Liu, Ramaswamy Chandrashekar, Mathew Eberts, Conference of the American^College^of^Veterinary^Internal^Medicine . 2016

机译：临床临床敏感性水平的临床差异，对抗体抗体的临床快速试验对ANPLASMA SPP的抗体。在狗
5. Coagulation Abnormalities in Ehrlichia canis-Infected Dogs and Detection and Dynamics of Anti-platelet Antibodies in Thrombocytopenic Dogs [D] . Shropshire, Sarah. 2018

机译：凝血性血小杂症犬肠梗理犬感染犬的凝血异常及抗血小板抗体的检测和动力学
6. Comparative Sensitivity of Gel-Diffusion and Tube Agglutination Tests for the Detection of Brucella canis Antibodies in Experimentally Infected Dogs [O] . Donald M. Myers, Víctor M. Varela-Díaz, Emilio A. Coltorti 1974

机译：凝胶扩散和管凝集试验检测实验感染犬布鲁氏菌抗体的敏感性比较
7. Comparative Sensitivity of Gel-Diffusion and Tube Agglutination Tests for the Detection of Brucella canis Antibodies in Experimentally Infected Dogs [O] . Donald M. Myers, Víctor M. Varela-Díaz, Emilio A. Coltorti 1974

机译：凝胶扩散和管凝集试验检测实验感染犬的Brucella Canis抗体的比较敏感性
8. Serosurvey for the Prevalence of Brucella canis Antibodies in Dogs in Central Ohio [R] . Pue, H. L. 1983

机译：serosurvey为在俄亥俄州中部的狗的Brucella canis抗体的普遍性

Comparative Sensitivity of Gel-Diffusion and Tube Agglutination Tests for the Detection of Brucella canis Antibodies in Experimentally Infected Dogs

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