Genetic Tool Development for a New Host for Biotechnology, the Thermotolerant Bacterium Bacillus coagulans

ákos T. Kovács; Mariska van Hartskamp; Oscar P. Kuipers; Richard van Kranenburg

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Genetic Tool Development for a New Host for Biotechnology, the Thermotolerant Bacterium Bacillus coagulans

机译：新型生物技术宿主耐热凝结芽孢杆菌的遗传工具开发

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Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre- lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.

机译：凝结芽孢杆菌作为可再生资源的平台化学品的工业生产有机体具有良好潜力，但可用的遗传工具有限。在这里，我们介绍了使用Crelox系统的靶向基因破坏系统，用于监视基因转录的LacZ报告基因检测方法的开发以及异源d-乳酸脱氢酶表达。

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《Applied Microbiology》 |2010年第12期|共4页
作者
ákos T. Kovács; Mariska van Hartskamp; Oscar P. Kuipers; Richard van Kranenburg;
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中图分类微生物学;
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1. Genetic Tool Development for a New Host for Biotechnology, the Thermotolerant Bacterium Bacillus coagulans [J] . ákos T. Kovács, Mariska van Hartskamp, Oscar P. Kuipers, Applied and Environmental Microbiology . 2010,第12期

机译：新型生物技术宿主耐热凝结芽孢杆菌的遗传工具开发
2. Complete Genome Sequence of a thermotolerant sporogenic lactic acid bacterium, Bacillus coagulans strain 36D1 [J] . Mun Su Rhee, Brélan E. Moritz, Gary Xie, Standards in Genomic Sciences . 2011,第3期

机译：耐热芽孢乳酸菌的完整基因组序列，凝结芽孢杆菌菌株36D1
3. Development of plasmid vector and electroporation condition for gene transfer in sporogenic lactic acid bacterium, Bacillus coagulans. [J] . Rhee MS, Kim JW, Qian Y, Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2007,第1期

机译：产孢乳酸菌凝结芽孢杆菌基因转移的质粒载体的开发和电穿孔条件。
4. Cloning and Sequence Analysis of a Partial L-Lactate Dehydrogenase Gene of Bacillus Coagulans Tl-4v: A Partial ldh gene of B. coagulans TL4 [C] . Binzhe Wang, Qiang Gao, Bing Liu, International Conference on Bioinformatics and Biomedical Technology . 2010

机译：芽孢杆菌T1-4V的部分L-乳酸脱氢酶基因的克隆及序列分析：B.CoAGulans TL4的部分LDH基因
5. Effects of Bacillus coagulans GBI-30, 6086 Probiotic Added to Extruded Pet Food on Apparent Nutrient Digestibility, Stool Quality, Intestinal Health Indicators, and Fecal Microbiota of Healthy Adult Dogs [D] . Acuff, Heather Lynn. 2021

机译：芽孢杆菌凝血蛋白GBI-30,6086益生菌对健康成年犬的表观营养消化率，粪便质量，肠道健康指标和粪便微生物的挤出宠物食品中加入挤出的宠物食品
6. Genetic Tool Development for a New Host for Biotechnology the Thermotolerant Bacterium Bacillus coagulans [O] . Ákos T. Kovács, Mariska van Hartskamp, Oscar P. Kuipers, 2010

机译：遗传工具的开发为生物技术的新宿主即耐热芽孢杆菌凝结芽孢杆菌
7. Genetic Tool Development for a New Host for Biotechnology, the Thermotolerant Bacterium Bacillus coagulans [O] . Kovacs Akos T., van Hartskamp Mariska, Kuipers Oscar P., 2010

机译：遗传工具的开发为生物技术的新宿主，即耐热芽孢杆菌凝结芽孢杆菌
8. Control of Bacillus stearothermophilus and Bacillus coagulans in Low-Acid Foodwith Food-Grade Additives [R] . Sikes, A. 1995

机译：用食品级添加剂控制低酸食品中的嗜热脂肪芽孢杆菌和凝结芽孢杆菌

Genetic Tool Development for a New Host for Biotechnology, the Thermotolerant Bacterium Bacillus coagulans

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