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首页> 外文期刊>Applied Microbiology >Estimation of Uptake of Humic Substances from Different Sources by Escherichia coli Cells under Optimum and Salt Stress Conditions by Use of Tritium-Labeled Humic Materials
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Estimation of Uptake of Humic Substances from Different Sources by Escherichia coli Cells under Optimum and Salt Stress Conditions by Use of Tritium-Labeled Humic Materials

机译：使用Use标记的腐殖质材料在最佳和盐胁迫条件下估算大肠杆菌细胞从不同来源摄取的腐殖质

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摘要

The primary goal of this paper is to demonstrate potential strengths of the use of tritium-labeled humic substances (HS) to quantify their interaction with living cells under various conditions. A novel approach was taken to study the interaction between a model microorganism and the labeled humic material. The bacterium Escherichia coli was used as a model microorganism. Salt stress was used to study interactions of HS with living cells under nonoptimum conditions. Six tritium-labeled samples of HS originating from coal, peat, and soil were examined. To quantify their interaction with E. coli cells, bioconcentration factors (BCF) were calculated and the amount of HS that penetrated into the cell interior was determined, and the liquid scintillation counting technique was used as well. The BCF values under optimum conditions varied from 0.9 to 13.1 liters kg~(?1) of cell biomass, whereas under salt stress conditions the range of corresponding values increased substantially and accounted for 0.2 to 130 liters kg~(?1). The measured amounts of HS that penetrated into the cells were 23 to 167 mg and 25 to 465 mg HS per kg of cell biomass under optimum and salt stress conditions, respectively. This finding indicated increased penetration of HS into E. coli cells under salt stress.
机译：本文的主要目的是证明使用tri标记的腐殖质（HS）来量化其在各种条件下与活细胞的相互作用的潜在优势。采取了一种新颖的方法来研究模型微生物和标记的腐殖质之间的相互作用。大肠杆菌被用作模型微生物。盐胁迫用于研究非最佳条件下HS与活细胞的相互作用。检验了六个of标记的HS样品，这些样品源自煤，泥煤和土壤。为了量化它们与大肠杆菌细胞的相互作用，计算了生物浓缩因子（BCF），并确定了渗透到细胞内部的HS的量，并且还使用了液体闪烁计数技术。最佳条件下的BCF值在0.9至13.1升kg〜（？1）之间变化，而在盐胁迫条件下，相应值的范围显着增加，占0.2至130升kg〜（？1）。在最佳和盐胁迫条件下，渗透到细胞中的HS的测量量分别为每千克细胞生物量23至167 mg和25至465 mg HS。该发现表明在盐胁迫下HS对大肠杆菌细胞的渗透增加。

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