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首页> 外文期刊>Applied Microbiology >Genomic and Functional Analyses of Rhodococcus equi Phages ReqiPepy6, ReqiPoco6, ReqiPine5, and ReqiDocB7
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Genomic and Functional Analyses of Rhodococcus equi Phages ReqiPepy6, ReqiPoco6, ReqiPine5, and ReqiDocB7

机译：马氏红球菌噬菌体ReqiPepy6，ReqiPoco6，ReqiPine5和ReqiDocB7的基因组和功能分析

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The isolation and results of genomic and functional analyses of Rhodococcus equi phages ReqiPepy6, ReqiDocB7, ReqiPine5, and ReqiPoco6 (hereafter referred to as Pepy6, DocB7, Pine5, and Poco6, respectively) are reported. Two phages, Pepy6 and Poco6, more than 75% identical, exhibited genome organization and protein sequence likeness to Lactococcus lactis phage 1706 and clostridial prophage elements. An unusually high fraction, 27%, of Pepy6 and Poco6 proteins were predicted to possess at least one transmembrane domain, a value much higher than the average of 8.5% transmembrane domain-containing proteins determined from a data set of 36,324 phage protein entries. Genome organization and protein sequence comparisons place phage Pine5 as the first nonmycobacteriophage member of the large Rosebush cluster. DocB7, which had the broadest host range among the four isolates, was not closely related to any phage or prophage in the database, and only 23 of 105 predicted encoded proteins could be assigned a functional annotation. Because of the relationship of Rhodococcus to Mycobacterium , it was anticipated that these phages should exhibit some of the features characteristic of mycobacteriophages. Traits that were identified as shared by the Rhodococcus phages and mycobacteriophages include the prevalent long-tailed morphology and the presence of genes encoding LysB-like mycolate-hydrolyzing lysis proteins. Application of DocB7 lysates to soils amended with a host strain of R. equi reduced recoverable bacterial CFU, suggesting that phage may be useful in limiting R. equi load in the environment while foals are susceptible to infection.
机译：报告了红球菌噬菌体ReqiPepy6，ReqiDocB7，ReqiPine5和ReqiPoco6（以下分别称为Pepy6，DocB7，Pine5和Poco6）的分离以及基因组和功能分析的结果。两个噬菌体，Pepy6和Poco6，具有超过75％的相同性，与乳酸乳球菌噬菌体1706和梭菌属噬菌体元件相比，具有基因组组织和蛋白质序列相似性。据预测，Pepy6和Poco6蛋白的异常高比例（27％）具有至少一个跨膜结构域，该值远远高于从36,324个噬菌体蛋白条目的数据集确定的含8.5％跨膜结构域蛋白的平均值。基因组组织和蛋白质序列比较使噬菌体Pine5成为大型Rosebush簇的第一个非分枝杆菌噬菌体成员。 DocB7在这四个分离株中具有最广泛的宿主范围，与数据库中的任何噬菌体或噬菌体都没有密切关系，并且在105个预测的编码蛋白中只有23个可以分配功能注释。由于红球菌与分枝杆菌的关系，预计这些噬菌体应表现出分枝杆菌噬菌体的某些特征。被鉴定为由红球菌噬菌体和分枝杆菌噬菌体共有的特征包括普遍的长尾形态和编码LysB样霉菌酸酯水解裂解蛋白的基因的存在。将DocB7裂解物应用于改良了R. equi还原性可回收细菌CFU的宿主菌株的土壤，这表明噬菌体可用于限制环境中的R. equi负载，而小马驹易于感染。

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