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首页> 外文期刊>Applied Microbiology >Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions
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Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

机译：嗜热志贺氏菌G3的分离和鉴定，能够在中温条件下有效进行纤维素糖化

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A novel Shigella strain ( Shigella flexneri G3) showing high cellulolytic activity under mesophilic, anaerobic conditions was isolated and characterized. The bacterium is Gram negative, short rod shaped, and nonmotile and displays effective production of glucose, cellobiose, and other oligosaccharides from cellulose (Avicel PH-101) under optimal conditions (40°C and pH 6.5). Approximately 75% of the cellulose was hydrolyzed in modified ATCC 1191 medium containing 0.3% cellulose, and the oligosaccharide production yield and specific production rate reached 375 mg g Avicel~(?1) and 6.25 mg g Avicel~(?1) h~(?1), respectively, after a 60-hour incubation. To our knowledge, this represents the highest oligosaccharide yield and specific rate from cellulose for mesophilic bacterial monocultures reported so far. The results demonstrate that S. flexneri G3 is capable of rapid conversion of cellulose to oligosaccharides, with potential biofuel applications under mesophilic conditions.
机译：分离并鉴定了在嗜温厌氧条件下显示高纤维素分解活性的新型志贺氏菌菌株（弗氏志贺氏菌G3）。该细菌为革兰氏阴性，短杆状且不活动，在最佳条件（40°C和pH 6.5）下，能从纤维素（Avicel PH-101）中有效产生葡萄糖，纤维二糖和其他寡糖。大约75％的纤维素在含有0.3％纤维素的改良ATCC 1191培养基中水解，寡糖的产量和比产率分别达到375 mg g Avicel〜（？1）和6.25 mg g Avicel〜（？1）h〜（孵育60小时后，分别取？1）。据我们所知，这代表了迄今为止报道的嗜温细菌单培养的最高寡糖产率和纤维素的特定比率。结果表明，弗氏链球菌G3能够将纤维素快速转化为寡糖，在中温条件下具有潜在的生物燃料应用。

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