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首页> 外文期刊>Applied Microbiology >Pulsed-Field Gel Electrophoresis Supports the Presence of Host-Adapted Salmonella enterica subsp. enterica Serovar Typhimurium Strains in the British Garden Bird Population
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Pulsed-Field Gel Electrophoresis Supports the Presence of Host-Adapted Salmonella enterica subsp. enterica Serovar Typhimurium Strains in the British Garden Bird Population

机译：脉冲场凝胶电泳支持宿主适应性沙门氏菌肠亚种的存在。英国花园鸟类种群中的肠型血清型鼠伤寒菌株

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Salmonellosis is a frequently diagnosed infectious disease of passerine birds in garden habitats within Great Britain with potential implications for human and domestic animal health. Postmortem examinations were performed on 1,477 garden bird carcasses of circa 50 species from England and Wales, 1999 to 2007 inclusive. Salmonellosis was confirmed in 263 adult birds of 10 passerine species in this 11-year longitudinal study. A subset of 124 fully biotyped Salmonella enterica subsp. enterica serovar Typhimurium isolates was examined using pulsed-field gel electrophoresis to investigate the hypothesis that these strains are host adapted and to determine whether this molecular technique offers greater resolution in understanding the epidemiology of Salmonella Typhimurium infection than phage typing alone. For the two most common phage types, definitive type (DT) 40 and DT56v, which together accounted for 97% (120/124) of isolates, pulsed-field gel electrophoresis groupings closely correlated with phage type with remarkably few exceptions. A high degree of genetic similarity (>90%) was observed within and between the two most common pulsed-field gel electrophoresis groups. No clustering or variation was found in the pulsed-field gel electrophoresis groupings by bird species, year, or geographical region beyond that revealed by phage typing. These findings support the hypothesis that there are currently two host-adapted Salmonella phage types, S . Typhimurium DT40 and DT56v, circulating widely in British garden birds and that the reservoir of infection is maintained within wild bird populations. Large-scale multilocus sequence typing studies are required to further investigate the epidemiology of this infection.
机译：沙门氏菌病是在英国境内花园栖息地中经常被诊断为雀形目鸟类的传染病，对人类和家畜的健康具有潜在的影响。在1999年至2007年期间，对英格兰和威尔士的大约50种物种的1477种花园鸟尸体进行了尸检。在这项为期11年的纵向研究中，沙门氏菌病已在10种雀形目的263只成年鸟类中得到确认。 124个完全生物型肠炎沙门氏菌亚种的子集。使用脉冲场凝胶电泳检查了肠炎血清鼠伤寒分离株，以调查这些菌株是否适应宿主的假说，并确定这种分子技术是否比单独的噬菌体分型在了解沙门氏菌感染的流行病学方面提供了更高的分辨率。对于两种最常见的噬菌体类型，定型（DT）40和DT56v占分离物的97％（120/124），脉冲场凝胶电泳分组与噬菌体类型密切相关，只有极少数例外。在两个最常见的脉冲场凝胶电泳组之间及其之间观察到高度的遗传相似性（> 90％）。在脉冲场凝胶电泳分组中，未发现鸟种，年份或地理区域超出通过噬菌体分型显示的聚类或变化。这些发现支持以下假设：目前存在两种适应宿主的沙门氏菌噬菌体类型S。鼠伤寒DT40和DT56v在英国花园鸟类中广泛传播，并且感染的库在野生鸟类种群中维持。需要大规模多基因座序列分型研究来进一步研究这种感染的流行病学。

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