Rapid Screening Method for Compounds That Affect the Growth and Germination of Candida albicans, Using a Real-Time PCR Thermocycler

Lucja M. Jarosz; Bastiaan P. Krom

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Rapid Screening Method for Compounds That Affect the Growth and Germination of Candida albicans, Using a Real-Time PCR Thermocycler

机译：使用实时PCR热循环仪快速筛选影响白色念珠菌生长和发芽的化合物的方法

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We propose a screening method for compounds affecting growth and germination in Candida albicans using a real-time PCR thermocycler to quantify green fluorescent protein (GFP) fluorescence. Using P _(ACT1)-GFP and P _(HWP1)-GFP reporter strains, the effects of a wide range of compounds on growth and hyphal formation were quantitatively assessed within 3 h after inoculation.

机译：我们提出了一种筛选化合物的方法，该化合物使用实时PCR热循环仪对白色念珠菌的生长和萌发产生影响，以量化绿色荧光蛋白（GFP）荧光。使用P_（ACT1）-GFP和P_（HWP1）-GFP报告菌株，在接种后3小时内定量评估了多种化合物对生长和菌丝形成的影响。

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《Applied Microbiology》 |2011年第22期|共4页
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Lucja M. Jarosz; Bastiaan P. Krom;
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中图分类微生物学;
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1. Rapid Screening Method for Compounds That Affect the Growth and Germination of Candida albicans, Using a Real-Time PCR Thermocycler [J] . Lucja M. Jarosz, Bastiaan P. Krom Applied and Environmental Microbiology . 2011,第22期

机译：使用实时PCR热循环仪快速筛选影响白色念珠菌生长和发芽的化合物的方法
2. Rapid and Accurate Identification of Candida albicans and Candida dubliniensis by Real-Time PCR and Melting Curve Analysis [J] . Asadzadeh M., Ahmad S., Al-Sweih N., Medical principles and practice: international journal of the Kuwait University, Health Science Centre . 2018,第6期

机译：通过实时PCR和熔化曲线分析，快速准确地鉴定念珠菌和念珠菌和念珠菌植物
3. Transcriptional profiling of the early stages of germination in Candida albicans by real-time RT-PCR [J] . Toyoda Mika, Cho Tamaki, Kaminishi Hidenori, FEMS Yeast Research . 2004,第3期

机译：实时RT-PCR对白色念珠菌发芽早期进行转录分析
4. Screening of Metabolites Secondary Compounds in Extract of Moringa Fruit and Determination of Inhibitory Effect on Growth of The Fungus Candida Albicans [C] . Siti Nuryanti, Dwi Juli Puspitasari International Conference on Research, Implementation, and Education of Mathematics and Science . 2017

机译：浅思岩中的代谢物中学化合物及抑制对真菌念珠菌生长的抑制作用
5. Rapid and sensitive detection of aflatoxin in animal feeds and food grains using immunomagnetic bead based recovery and real-time immuno quantitative PCR (RT-iqPCR) assay. [D] . Babu, Dinesh. 2010

机译：使用基于免疫磁珠的回收率和实时免疫定量PCR（RT-iqPCR）分析快速，灵敏地检测动物饲料和粮食中的黄曲霉毒素。
6. Rapid Screening Method for Compounds That Affect the Growth and Germination of Candida albicans Using a Real-Time PCR Thermocycler [O] . Lucja M. Jarosz, Bastiaan P. Krom 2011

机译：使用实时PCR热循环仪快速筛选影响白色念珠菌生长和发芽的化合物的方法
7. Rapid Screening Method for Compounds That Affect the Growth and Germination of Candida albicans, Using a Real-Time PCR Thermocycler [O] . Jarosz, Lucja M., Krom, Bastiaan P. 2011

机译：使用实时PCR热循环仪快速筛选影响白色念珠菌生长和发芽的化合物的方法

Rapid Screening Method for Compounds That Affect the Growth and Germination of Candida albicans, Using a Real-Time PCR Thermocycler

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