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首页> 外文期刊>Applied Microbiology >Unexpected Stability of Bacteroidetes and Firmicutes Communities in Laboratory Biogas Reactors Fed with Different Defined Substrates
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Unexpected Stability of Bacteroidetes and Firmicutes Communities in Laboratory Biogas Reactors Fed with Different Defined Substrates

机译：饲喂不同定义底物的实验室沼气反应器中拟杆菌和菌丝群落的意外稳定性

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摘要

In the present study, bacterial communities in 200-liter biogas reactors containing liquid manure consecutively fed with casein, starch, and cream were investigated over a period of up to 33 days. A 16S rRNA gene clone library identified Bacteroidetes and Firmicutes as the most abundant bacterial groups in the starting material, at 58.9% and 30.1% of sequences, respectively. The community development of both groups was monitored by real-time PCR and single-strand conformation polymorphism (SSCP) analysis. The Firmicutes and Bacteroidetes communities were unexpectedly stable and hardly influenced by batch-feeding events. The continuous feeding of starch led to community shifts that nevertheless contributed to a stable reactor performance. A longer starving period and a change in the pH value resulted in further community shifts within the Bacteroidetes but did not influence the Firmicutes . Predominant DNA bands from SSCP gels were cloned and sequenced. Sequences related to Peptococcaceae , Cytophagales , and Petrimonas sulfuriphila were found in all samples from all experiments. Real-time PCR demonstrated the abundance of members of the phylum Bacteroidetes and also reflected changes in gene copy numbers in conjunction with a changing pH value and acetate accumulation.
机译：在本研究中，在长达33天的时间内研究了200升沼气反应器中的细菌群落，该反应器中包含连续饲喂酪蛋白，淀粉和奶油的液态肥料。 16S rRNA基因克隆文库鉴定出拟杆菌属和菌类是起始材料中最丰富的细菌群，分别占序列的58.9％和30.1％。通过实时PCR和单链构象多态性（SSCP）分析监测两组的社区发展。 Firmicutes和Bacteroidetes社区出乎意料地稳定，几乎不受批量进食事件的影响。淀粉的连续进料导致群落转移，但仍有助于稳定的反应器性能。更长的饥饿期和pH值的变化导致拟杆菌内的群落进一步转移，但并不影响硬壳菌。克隆并测序了来自SSCP凝胶的主要DNA条带。在所有实验的所有样品中均发现了与肽球菌科，噬菌体和嗜盐小球藻相关的序列。实时PCR证明了拟杆菌门的成员丰富，并且还反映了基因拷贝数的变化以及pH值和乙酸盐积累的变化。

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