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Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales

机译：精细地理尺度上炭疽杆菌土壤分离物的遗传多样性

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Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison ( Bison bison ) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from these samples and confirmed using phenotypic characterization and real-time PCR. Confirmed B. anthracis isolates were typed using multiple-locus variable-number tandem repeat analysis (MLVA15) and single-nucleotide-repeat analysis (SNRA). B. anthracis isolates split into two clades based on MLVA15, while SNRA allowed some isolates between carcass sites to be distinguished from each other. SNRA polymorphisms were also present within a single carcass site. Some isolates from different carcass sites having the same SNRA type had divergent MLVA types; this finding leads to questions about hierarchical typing methods and the robustness of the fine-scale typing of Bacillus anthracis .
机译：在2000年和2001年炭疽热暴发期间和之后，从尸体现场收集了环境样品，这些样品来自伍德布法罗国家公园和伍德布法罗国家公园以北的胡克湖地区的野牛（野牛野牛）种群。从这些样品中分离出炭疽芽孢杆菌孢子，并使用表型表征和实时PCR进行确认。使用多位点可变数串联重复分析（MLVA15）和单核苷酸重复分析（SNRA）对确诊的炭疽芽孢杆菌分离株进行分型。炭疽芽孢杆菌分离株根据MLVA15分为两个进化枝，而SNRA允许将car体部位之间的某些分离株区分开。 SNRA多态性也存在于单个car体部位。来自不同A体部位且具有相同SNRA类型的某些分离株具有不同的MLVA类型;其他的分离株具有不同的MLVA类型。这一发现引发了有关分层分型方法和炭疽芽孢杆菌精细分型的鲁棒性的问题。

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