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首页> 外文期刊>Applied Microbiology >Seasonal Diversity of Planktonic Protists in Southwestern Alberta Rivers over a 1-Year Period as Revealed by Terminal Restriction Fragment Length Polymorphism and 18S rRNA Gene Library Analyses
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Seasonal Diversity of Planktonic Protists in Southwestern Alberta Rivers over a 1-Year Period as Revealed by Terminal Restriction Fragment Length Polymorphism and 18S rRNA Gene Library Analyses

机译：终端限制片段长度多态性和18S rRNA基因文库分析揭示了艾伯塔省西南部浮游生物在1年内的季节性多样性

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The temporal dynamics of planktonic protists in river water have received limited attention despite their ecological significance and recent studies linking phagotrophic protists to the persistence of human-pathogenic bacteria. Using molecular-based techniques targeting the 18S rRNA gene, we studied the seasonal diversity of planktonic protists in Southwestern Alberta rivers (Oldman River Basin) over a 1-year period. Nonmetric multidimensional scaling analysis of terminal restriction fragment length polymorphism (T-RFLP) data revealed distinct shifts in protistan community profiles that corresponded to season rather than geographical location. Community structures were examined by using clone library analysis; HaeIII restriction profiles of 18S rRNA gene amplicons were used to remove prevalent solanaceous plant clones prior to sequencing. Sanger sequencing of the V1-to-V3 region of the 18S rRNA gene libraries from spring, summer, fall, and winter supported the T-RFLP results and showed marked seasonal differences in the protistan community structure. The spring library was dominated by Chloroplastidae (29.8%), Centrohelida (28.1%), and Alveolata (25.5%), while the summer and fall libraries contained primarily fungal clones (83.0% and 88.0%, respectively). Alveolata (35.6%), Euglenozoa (24.4%), Chloroplastida (15.6%), and Fungi (15.6%) dominated the winter library. These data demonstrate that planktonic protists, including protozoa, are abundant in river water in Southwestern Alberta and that conspicuous seasonal shifts occur in the community structure.
机译：尽管具有生态学意义，但河水中浮游生物的时空动态受到了有限的关注，最近的研究将吞噬营养的生物与人类致病细菌的持续存在联系起来。使用针对18S rRNA基因的基于分子的技术，我们研究了西南阿尔伯塔省河流（奥尔德曼河流域）一年内浮游生物的季节性多样性。终端限制片段长度多态性（T-RFLP）数据的非度量多维标度分析显示，对应于季节而不是地理位置的protistan群落概况发生了明显变化。通过克隆文库分析检查了群落结构;在测序之前，使用18S rRNA基因扩增子的HaeIII限制性酶切图谱去除普遍的茄科植物克隆。从春季，夏季，秋季和冬季对18S rRNA基因文库的V1-to-V3区域进行Sanger测序，支持了T-RFLP结果，并显示了Protistan群落结构的明显季节性差异。春季文库主要由叶绿体科（29.8％），中心螺旋菌（28.1％）和肺泡纲（25.5％）主导，而夏季和秋季文库主要包含真菌克隆（分别为83.0％和88.0％）。冬季文库中主要有小叶藻（35.6％），裸藻（24.4％），叶绿体（15.6％）和真菌（15.6％）。这些数据表明西南阿尔伯塔省河水中的浮游生物（包括原生动物）丰富，并且群落结构发生明显的季节性变化。

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