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首页> 外文期刊>Applied Microbiology >Detection of Achromobacter xylosoxidans in Hospital, Domestic, and Outdoor Environmental Samples and Comparison with Human Clinical Isolates
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Detection of Achromobacter xylosoxidans in Hospital, Domestic, and Outdoor Environmental Samples and Comparison with Human Clinical Isolates

机译：医院，家庭和室外环境样品中木糖氧化无色杆菌的检测及其与人类临床分离株的比较

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Achromobacter xylosoxidans is an aerobic nonfermentative Gram-negative rod considered an important emerging pathogen among cystic fibrosis (CF) patients worldwide and among immunocompromised patients. This increased prevalence remains unexplained, and to date no environmental reservoir has been identified. The aim of this study was to identify potential reservoirs of A. xylosoxidans in hospital, domestic, and outdoor environments and to compare the isolates with clinical ones. From 2011 to 2012, 339 samples were collected in Dijon's university hospital, in healthy volunteers' homes in the Dijon area, and in the outdoor environment in Burgundy (soil, water, mud, and plants). We designed a protocol to detect A. xylosoxidans in environmental samples based on a selective medium: MCXVAA (MacConkey agar supplemented with xylose, vancomycin, aztreonam, and amphotericin B). Susceptibility testing, genotypic analysis by pulsed-field gel electrophoresis, and bla _(OXA-114) sequencing were performed on the isolates. A total of 50 strains of A. xylosoxidans were detected in hospital (33 isolates), domestic (9 isolates), and outdoor (8 isolates) samples, mainly in hand washing sinks, showers, and water. Most of them were resistant to ciprofloxacin (49 strains). Genotypic analysis and bla _(OXA-114) sequencing revealed a wide diversity among the isolates, with 35 pulsotypes and 18 variants of oxacillinases. Interestingly, 10 isolates from hospital environment were clonally related to clinical isolates previously recovered from hospitalized patients, and one domestic isolate was identical to one recovered from a CF patient. These results indicate that A. xylosoxidans is commonly distributed in various environments and therefore that CF patients or immunocompromised patients are surrounded by these reservoirs.
机译：木氧化无色杆菌是一种需氧性非发酵性革兰氏阴性棒，被认为是全世界囊性纤维化（CF）患者和免疫功能低下患者的重要新兴病原体。这种增加的患病率仍无法解释，迄今为止，尚未发现任何环境库。这项研究的目的是在医院，家庭和室外环境中鉴定木糖氧化过氧化物酶的潜在储库，并将其与临床菌株进行比较。从2011年到2012年，在第戎大学医院，第戎地区健康的志愿者之家以及勃艮第的室外环境（土壤，水，泥土和植物）中收集了339个样本。我们设计了一种协议，用于基于选择性培养基：MCXVAA（补充有木糖，万古霉素，氨曲南和两性霉素B的MacConkey琼脂）来检测环境样品中的木糖氧化杆菌。对分离物进行了药敏试验，脉冲场凝胶电泳的基因型分析和bla_（OXA-114）测序。在医院（33个分离株），家庭（9个分离株）和室外（8个分离株）样品中，主要在洗手池，淋浴器和水中，共检出50株木糖氧化杆菌。他们中的大多数对环丙沙星有抗药性（49株）。基因型分析和bla_（OXA-114）测序揭示了分离株之间的广泛差异，其中包括35种脉冲型和18种变异的草酸酶。有趣的是，有10株来自医院环境的分离株与先前从住院患者中回收的临床分离株有克隆关系，其中一种家庭分离株与从CF患者中回收的分离株相同。这些结果表明木糖氧化过氧化物酶通常分布在各种环境中，因此，CF患者或免疫功能低下的患者被这些储库所包围。

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