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首页> 外文期刊>Applied Microbiology >Engineered Escherichia coli with Periplasmic Carbonic Anhydrase as a Biocatalyst for CO2 Sequestration
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Engineered Escherichia coli with Periplasmic Carbonic Anhydrase as a Biocatalyst for CO2 Sequestration

机译：工程化大肠杆菌，具有周质碳酸酐酶作为二氧化碳封存的生物催化剂

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Carbonic anhydrase is an enzyme that reversibly catalyzes the hydration of carbon dioxide (CO_(2)). It has been suggested recently that this remarkably fast enzyme can be used for sequestration of CO_(2), a major greenhouse gas, making this a promising alternative for chemical CO_(2) mitigation. To promote the economical use of enzymes, we engineered the carbonic anhydrase from Neisseria gonorrhoeae ( ng CA) in the periplasm of Escherichia coli , thereby creating a bacterial whole-cell catalyst. We then investigated the application of this system to CO_(2) sequestration by mineral carbonation, a process with the potential to store large quantities of CO_(2). ng CA was highly expressed in the periplasm of E. coli in a soluble form, and the recombinant bacterial cell displayed the distinct ability to hydrate CO_(2) compared with its cytoplasmic ng CA counterpart and previously reported whole-cell CA systems. The expression of ng CA in the periplasm of E. coli greatly accelerated the rate of calcium carbonate (CaCO_(3)) formation and exerted a striking impact on the maximal amount of CaCO_(3) produced under conditions of relatively low pH. It was also shown that the thermal stability of the periplasmic enzyme was significantly improved. These results demonstrate that the engineered bacterial cell with periplasmic ng CA can successfully serve as an efficient biocatalyst for CO_(2) sequestration.
机译：碳酸酐酶是可逆地催化二氧化碳水合的酶（CO_（2））。最近有人提出，这种非常快的酶可以用于隔离主要的温室气体CO_（2），这使其成为缓解化学CO_（2）的有希望的替代方法。为了促进酶的经济使用，我们在大肠杆菌的周质中设计了淋病奈瑟氏球菌（ng CA）的碳酸酐酶，从而创建了细菌全细胞催化剂。然后，我们研究了该系统在通过矿物碳酸化作用隔离CO_（2）的过程中的应用，该过程具有存储大量CO_（2）的潜力。 ng CA在大肠杆菌的周质中以可溶形式高表达，并且重组细菌细胞与其胞质ng CA对应物和先前报道的全细胞CA系统相比，具有水合CO_（2）的独特能力。在大肠杆菌的周质中ng CA的表达大大加快了碳酸钙（CaCO_（3））的形成速度，并对pH值相对较低的条件下产生的最大CaCO_（3）量产生了显着影响。还显示周质酶的热稳定性显着提高。这些结果表明，具有周质ng CA的工程细菌细胞可以成功地用作CO_（2）隔离的有效生物催化剂。

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