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首页> 外文期刊>Applied Microbiology >Stationary Phase and Nutrient Levels Trigger Transcription of a Genomic Locus Containing a Novel Peptide (TM1316) in the Hyperthermophilic Bacterium Thermotoga maritima
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Stationary Phase and Nutrient Levels Trigger Transcription of a Genomic Locus Containing a Novel Peptide (TM1316) in the Hyperthermophilic Bacterium Thermotoga maritima

机译：固定相和营养水平触发了在超嗜热细菌马氏体中含有新肽（TM1316）的基因座的转录。

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The genome of the hyperthermophilic bacterium Thermotoga maritima encodes numerous putative peptides/proteins of 100 amino acids or less. While most of these open reading frames (ORFs) are transcribed during growth, their corresponding physiological roles are largely unknown. The onset of stationary phase in T. maritima was accompanied by significant morphological changes and upregulation of several ORFs located in the TM1298-TM1336 genome locus. This region contains putative HicAB toxin-antitoxin pairs, hypothetical proteins, radical S -adenosylmethionine (SAM) enzymes, and ABC transporters. Of particular note was the TM1315-TM1319 operon, which includes a putative 31-amino-acid peptide (TM1316) that was the most highly transcribed gene in the transcriptome during stationary phase. Antibodies directed against a synthetic version of TM1316 were used to track its production, which correlated closely with transcriptomic data. Immunofluorescence microscopy revealed that TM1316 was localized to the cell envelope and prominent in cell aggregates formed during stationary phase. The only functionally characterized locus with an organization similar to that of TM1315-TM1319 is in Bacillus subtilis , which contains subtilosin A, a cyclic peptide with Cys–to–α-carbon linkages that functions as an antilisterial bacteriocin. While the organization of TM1316 resembled that of the Bacillus peptide (e.g., in its number of amino acids and spacing of Cys residues), preparations containing high levels of TM1316 affected the growth of neither Thermotoga species nor Pyrococcus furiosus , a hyperthermophilic archaeon isolated from the same locale as T. maritima . Several other putative Cys-rich peptides could be identified in the TM1298-TM1336 locus, and while their roles are also unclear, they merit examination as potential antimicrobial agents in hyperthermophilic biotopes.
机译：嗜热嗜热菌马氏菌（Thermotoga maritima）的基因组编码许多100个或更少氨基酸的推定肽/蛋白质。尽管大多数开放阅读框（ORF）在生长过程中均被转录，但其相应的生理作用在很大程度上尚不清楚。海藻T.平稳期的开始伴随着明显的形态变化和位于TM1298-TM1336基因组基因座中的几个ORF的上调。该区域包含推定的HicAB毒素-抗毒素对，假设的蛋白质，自由基S-腺苷甲硫氨酸（SAM）酶和ABC转运蛋白。特别值得注意的是TM1315-TM1319操纵子，它包含一个推定的31个氨基酸的肽段（TM1316），该肽段是静止期转录组中转录度最高的基因。使用针对TM1316合成版本的抗体来跟踪其产生，该产物与转录组数据密切相关。免疫荧光显微镜检查显示TM1316定位于细胞包膜，并在固定期形成的细胞聚集体中突出。具有类似于TM1315-TM1319的组织的唯一具有功能特征的基因座是在枯草芽孢杆菌中，其包含枯草杆菌蛋白酶A，枯草杆菌蛋白酶A是具有Cys-α-碳键的环状肽，起抗李斯特菌的作用。虽然TM1316的组织类似于芽孢杆菌肽的组织（例如，其氨基酸数量和Cys残基的间隔），但含有高水平TM1316的制剂既不影响Thermotoga菌种又不影响激烈热球菌Pyrococcus furiosus的生长。与滨海T.相同的地区。可以在TM1298-TM1336基因座中鉴定出其他几种推定的富含Cys的肽，尽管它们的作用也不清楚，但值得检查，它们可作为超嗜热生物群落中潜在的抗菌剂。

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