...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Geobacillus thermoglucosidasius Endospores Function as Nuclei for the Formation of Single Calcite Crystals
【24h】

Geobacillus thermoglucosidasius Endospores Function as Nuclei for the Formation of Single Calcite Crystals

机译：Geobacillus thermoglucosidasius内生孢子可作为核形成单个方解石晶体

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Geobacillus thermoglucosidasius colonies were placed on an agar hydrogel containing acetate, calcium ions, and magnesium ions, resulting in the formation of single calcite crystals (calcites) within and peripheral to the plating area or parent colony. Microscopic observation of purified calcites placed on the surface of soybean casein digest (SCD) nutrient medium revealed interior crevices from which bacterial colonies originated. Calcites formed on the gel contained [1-~(13)C]- and [2-~(13)C]acetate, demonstrating that G. thermoglucosidasius utilizes carbon derived from acetate for calcite formation. During calcite formation, vegetative cells swam away from the parent colony in the hydrogel. Hard-agar hydrogel inhibited the formation of calcites peripheral to the parent colony. The calcite dissolved completely in 1 M HCl, with production of bubbles, and the remaining endospore-like particles were easily stained with Brilliant green dye. The presence of DNA and protein in calcites was demonstrated by electrophoresis. We propose that endospores initiate the nucleation of calcites. Endospores of G. thermoglucosidasius remain alive and encapsulated in calcites.
机译：将热葡糖芽孢杆菌（Geobacillus thermoglucosidasius）菌落放在含有乙酸盐，钙离子和镁离子的琼脂水凝胶上，导致在平板区域或亲本菌落内和外围形成单个方解石晶体（方解石）。放置在大豆酪蛋白消化（SCD）营养培养基表面上的纯化方解石的显微镜观察显示出内部菌落是细菌菌落的起源。凝胶上形成的方解石含有[1-〜（13）C]-和[2-〜（13）C]乙酸盐，这表明热葡萄糖假丝酵母利用衍生自乙酸盐的碳来形成方解石。在方解石形成过程中，营养细胞游离了水凝胶中的母体集落。硬琼脂水凝胶抑制了亲本菌落周边的方解石形成。该方解石完全溶解在1 M HCl中，并产生气泡，剩余的类孢子状颗粒容易被Brilliant green染料染色。方解石中DNA和蛋白质的存在通过电泳证实。我们建议内生孢子引发方解石的成核。 G.thermoglucosidasius的内生孢子仍然活着，并包裹在方解石中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号