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首页> 外文期刊>Applied Microbiology >Discovery and Characterization of a 5-Hydroxymethylfurfural Oxidase from Methylovorus sp. Strain MP688
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Discovery and Characterization of a 5-Hydroxymethylfurfural Oxidase from Methylovorus sp. Strain MP688

机译：发现和表征5-羟甲基糠醛氧化酶。应变MP688

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摘要

In the search for useful and renewable chemical building blocks, 5-hydroxymethylfurfural (HMF) has emerged as a very promising candidate, as it can be prepared from sugars. HMF can be oxidized to 2,5-furandicarboxylic acid (FDCA), which is used as a substitute for petroleum-based terephthalate in polymer production. On the basis of a recently identified bacterial degradation pathway for HMF, candidate genes responsible for selective HMF oxidation have been identified. Heterologous expression of a protein from Methylovorus sp. strain MP688 in Escherichia coli and subsequent enzyme characterization showed that the respective gene indeed encodes an efficient HMF oxidase (HMFO). HMFO is a flavin adenine dinucleotide-containing oxidase and belongs to the glucose-methanol-choline-type flavoprotein oxidase family. Intriguingly, the activity of HMFO is not restricted to HMF, as it is active with a wide range of aromatic primary alcohols and aldehydes. The enzyme was shown to be relatively thermostable and active over a broad pH range. This makes HMFO a promising oxidative biocatalyst that can be used for the production of FDCA from HMF, a reaction involving both alcohol and aldehyde oxidations.
机译：在寻找有用的和可再生的化学结构单元时，5-羟甲基糠醛（HMF）已经成为非常有前途的候选物，因为它可以从糖中制得。 HMF可以被氧化为2,5-呋喃二甲酸（FDCA），可在聚合物生产中用作石油基对苯二甲酸酯的替代品。基于最近鉴定的HMF细菌降解途径，已经鉴定了负责选择性HMF氧化的候选基因。来自甲基甲虫的蛋白质的异源表达。大肠杆菌中的MP688菌株和随后的酶表征表明，相应的基因确实编码有效的HMF氧化酶（HMFO）。 HMFO是一种含有黄素腺嘌呤二核苷酸的氧化酶，属于葡萄糖-甲醇-胆碱型黄素蛋白氧化酶家族。有趣的是，HMFO的活性不仅限于HMF，因为它对多种芳族伯醇和醛具有活性。该酶显示出相对热稳定性，并在很宽的pH范围内具有活性。这使HMFO成为一种有前途的氧化生物催化剂，可用于由HMF生产FDCA的过程，该反应涉及醇和醛的氧化反应。

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