...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Development and Bias Assessment of a Method for Targeted Metagenomic Sequencing of Marine Cyanobacteria
【24h】

Development and Bias Assessment of a Method for Targeted Metagenomic Sequencing of Marine Cyanobacteria

机译：海洋蓝细菌靶向基因组测序的方法的开发和偏差评估

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Prochlorococcus and Synechococcus are the most abundant photosynthetic organisms in oligotrophic waters and responsible for a significant percentage of the earth's primary production. Here we developed a method for metagenomic sequencing of sorted Prochlorococcus and Synechococcus populations using a transposon-based library preparation technique. First, we observed that the cell lysis technique and associated amount of input DNA had an important role in determining the DNA library quality. Second, we found that our transposon-based method provided a more even coverage distribution and matched more sequences of a reference genome than multiple displacement amplification, a commonly used method for metagenomic sequencing. We then demonstrated the method on Prochlorococcus and Synechococcus field populations from the Sargasso Sea and California Current isolated by flow cytometric sorting and found clear environmentally related differences in ecotype distributions and gene abundances. In addition, we saw a significant correspondence between metagenomic libraries sequenced with our technique and regular sequencing of bulk DNA. Our results show that this targeted method is a viable replacement for regular metagenomic approaches and will be useful for identifying the biogeography and genome content of specific marine cyanobacterial populations.
机译：原绿球菌和合成球菌是贫营养水域中最丰富的光合生物，占地球初级生产的很大比例。在这里，我们开发了一种使用基于转座子的文库制备技术对原球菌和聚球菌种群进行宏基因组测序的方法。首先，我们观察到细胞裂解技术和相关的输入DNA量在确定DNA文库质量中具有重要作用。其次，我们发现，基于转座子的方法比多置换扩增（一种宏基因组测序的常用方法）提供了更均匀的覆盖范围，并匹配了更多的参考基因组序列。然后，我们通过流式细胞仪分选证明了从Sargasso海和加州洋流中原球菌和Synechococcus野外种群的分离方法，并发现了与生态型分布和基因丰度明显相关的环境差异。此外，我们发现用我们的技术测序的宏基因组文库与大批量DNA的常规测序之间存在显着的对应关系。我们的结果表明，这种靶向方法是常规宏基因组学方法的可行替代方法，将对确定特定海洋蓝细菌种群的生物地理学和基因组含量有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号