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首页> 外文期刊>Applied Microbiology >Composition of Bacterial Communities Associated with Aurelia aurita Changes with Compartment, Life Stage, and Population
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Composition of Bacterial Communities Associated with Aurelia aurita Changes with Compartment, Life Stage, and Population

机译：与金黄色葡萄球菌相关的细菌群落组成随室，生活阶段和种群的变化

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The scyphozoan Aurelia aurita is recognized as a key player in marine ecosystems and a driver of ecosystem change. It is thus intensely studied to address ecological questions, although its associations with microorganisms remain so far undescribed. In the present study, the microbiota associated with A. aurita was visualized with fluorescence in situ hybridization (FISH) analysis, and community structure was analyzed with respect to different life stages, compartments, and populations of A. aurita by 16S rRNA gene amplicon sequencing. We demonstrate that the composition of the A. aurita microbiota is generally highly distinct from the composition of communities present in ambient water. Comparison of microbial communities from different developmental stages reveals evidence for life stage-specific community patterns. Significant restructuring of the microbiota during strobilation from benthic polyp to planktonic life stages is present, arguing for a restructuring during the course of metamorphosis. Furthermore, the microbiota present in different compartments of the adult medusa (exumbrella mucus and gastric cavity) display significant differences, indicating body part-specific colonization. A novel Mycoplasma strain was identified in both compartment-specific microbiota and is most likely present inside the epithelium as indicated by FISH analysis of polyps, indicating potential endosymbiosis. Finally, comparison of polyps of different populations kept under the same controlled laboratory conditions in the same ambient water showed population-specific community patterns, most likely due the genetic background of the host. In conclusion, the presented data indicate that the associated microbiota of A. aurita may play important functional roles, e.g., during the life cycle.
机译：脊椎动物Aurelia aurita被公认为海洋生态系统的关键角色和生态系统变化的驱动力。因此，尽管它与微生物的联系至今仍未得到描述，但人们对其进行了深入的研究以解决生态问题。在本研究中，通过荧光原位杂交（FISH）可视化与金黄色葡萄球菌相关的微生物群，并通过16S rRNA基因扩增子序列分析了金黄色葡萄球菌的不同生命阶段，区室和种群的群落结构。我们证明，A。aurita微生物群的组成通常与环境水中存在的群落组成高度不同。来自不同发育阶段的微生物群落的比较揭示了生命阶段特定的群落模式的证据。存在从底栖息肉到浮游生命阶段的微生物重生过程中的重大重组，这是在变态过程中的重组。此外，存在于成年水母的不同区室（伞状粘液和胃腔）中的微生物群显示出显着差异，表明身体部位特异性定植。如对息肉的FISH分析所表明，在这两个区室特异性菌群中均发现了一种新的支原体菌株，该菌株很可能存在于上皮细胞内，表明潜在的共生作用。最后，在相同的受控实验室条件下，在相同的环境水中对不同种群的息肉进行比较，发现了特定种群的群落模式，这很可能是宿主的遗传背景所致。总之，所提供的数据表明，例如，金生命链球菌的相关微生物群可能在生命周期中起重要的功能作用。

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