...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Metabolic Impact of Redox Cofactor Perturbations on the Formation of Aroma Compounds in Saccharomyces cerevisiae
【24h】

Metabolic Impact of Redox Cofactor Perturbations on the Formation of Aroma Compounds in Saccharomyces cerevisiae

机译：氧化还原辅因子摄动对酿酒酵母中香气化合物形成的代谢影响。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Redox homeostasis is a fundamental requirement for the maintenance of metabolism, energy generation, and growth in Saccharomyces cerevisiae . The redox cofactors NADH and NADPH are among the most highly connected metabolites in metabolic networks. Changes in their concentrations may induce widespread changes in metabolism. Redox imbalances were achieved with a dedicated biological tool overexpressing native NADH-dependent or engineered NADPH-dependent 2,3-butanediol dehydrogenase, in the presence of acetoin. We report that targeted perturbation of the balance of cofactors (NAD~(+)/NADH or, to a lesser extent, NADP~(+)/NADPH) significantly affected the production of volatile compounds. In most cases, variations in the redox state of yeasts modified the formation of all compounds from the same biochemical pathway (isobutanol, isoamyl alcohol, and their derivatives) or chemical class (ethyl esters), irrespective of the cofactors. These coordinated responses were found to be closely linked to the impact of redox status on the availability of intermediates of central carbon metabolism. This was the case for α-keto acids and acetyl coenzyme A (acetyl-CoA), which are precursors for the synthesis of many volatile compounds. We also demonstrated that changes in the availability of NADH selectively affected the synthesis of some volatile molecules (e.g., methionol, phenylethanol, and propanoic acid), reflecting the specific cofactor requirements of the dehydrogenases involved in their formation. Our findings indicate that both the availability of precursors from central carbon metabolism and the accessibility of reduced cofactors contribute to cell redox status modulation of volatile compound formation.
机译：氧化还原稳态是维持酿酒酵母代谢，能量生成和生长的基本要求。氧化还原辅助因子NADH和NADPH是代谢网络中连接最紧密的代谢物。它们浓度的变化可能引起新陈代谢的广泛变化。氧化还原失衡是通过在乙酰丙酮存在下过表达天然NADH依赖或工程改造的NADPH依赖的2,3-丁二醇脱氢酶的专用生物学工具实现的。我们报告说，靶向干扰辅因子（NAD〜（+）/ NADH或较小程度的NADP〜（+）/ NADPH）平衡会显着影响挥发性化合物的生产。在大多数情况下，酵母的氧化还原状态变化会改变来自同一生化途径（异丁醇，异戊醇及其衍生物）或化学类别（乙酯）的所有化合物的形成，而与辅因子无关。发现这些协调的反应与氧化还原状态对中央碳代谢中间体的可用性有密切关系。 α-酮酸和乙酰辅酶A（乙酰辅酶A）就是这种情况，它们是许多挥发性化合物合成的前体。我们还证明了NADH可用性的变化选择性地影响了某些挥发性分子（例如，甲硫醇，苯乙醇和丙酸）的合成，反映了参与脱氢酶形成的特定辅因子要求。我们的发现表明，来自中央碳代谢的前体的可用性和减少的辅因子的可访问性都有助于挥发性化合物形成的细胞氧化还原状态调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号