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Immunization of Mice with Components of Pasteurella multocida

机译：用多杀性巴氏杆菌成分对小鼠进行免疫

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Log-phase cells of Pasteurella multocida strain P-1059 were used to prepare isolated culture filtrate, cell wall, and cytoplasmic components. Culture filtrate was further separated by column chromatography. A portion of cytoplasm and culture filtrate was conjugated to ferritin by means of metaxylylene diisocyanate. Cell walls induced more protection in mice than the conjugated or unconjugated cytoplasm or culture filtrate. The cell walls caused edema and erythema when given intradermally in rabbits, whereas cytoplasm and culture filtrate produced dermal necrosis. The first of four chromatographically separated fractions of culture filtrate was possibly more immunogenic in mice than cell walls. This fraction was less reactive intradermally in rabbits than cell walls but more reactive than the other fractions.
机译：多杀巴斯德氏菌P-1059菌株的对数期细胞用于制备分离的培养滤液，细胞壁和细胞质组分。通过柱色谱进一步分离培养滤液。通过间亚二甲苯基二异氰酸酯将一部分细胞质和培养物滤液与铁蛋白缀合。与结合或未结合的细胞质或培养滤液相比，细胞壁在小鼠中诱导了更多的保护作用。当给兔子皮内给予时，细胞壁引起水肿和红斑，而细胞质和培养物滤液产生真皮坏死。色谱分离的四种培养滤液中的第一种可能比细胞壁对小鼠的免疫原性更高。该级分在兔中的皮内反应性低于细胞壁，但比其他级分的反应性更高。

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