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首页> 外文期刊>Applied Microbiology >Integration of Insecticidal Protein Vip3Aa1 into Beauveria bassiana Enhances Fungal Virulence to Spodoptera litura Larvae by Cuticle and Per Os Infection
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Integration of Insecticidal Protein Vip3Aa1 into Beauveria bassiana Enhances Fungal Virulence to Spodoptera litura Larvae by Cuticle and Per Os Infection

机译：杀虫蛋白Vip3Aa1整合到球孢白僵菌中，通过角质层和Per Os感染增强了斜纹夜蛾幼虫的真菌毒力。

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The entomopathogenic fungus Beauveria bassiana acts slowly on insect pests through cuticle infection. Vegetative insecticidal proteins (Vip3A) of Bacillus thuringiensis kill lepidopteran pests rapidly, via per os infection, but their use for pest control is restricted to integration into transgenic plants. A transgenic B. bassiana strain (BbV28) expressing Vip3Aa1 (a Vip3A toxin) was thus created to infect the larvae of the oriental leafworm moth Spodoptera litura through conidial ingestion and cuticle adhesion. Vip3Aa1 (～88 kDa) was highly expressed in the conidial cytoplasm of BbV28 and was detected as a digested form (～62 kDa) in the larval midgut 18 and 36 h after conidial ingestion. The median lethal concentration (LC_(50)) of BbV28 against the second-instar larvae feeding on cabbage leaves sprayed with conidial suspensions was 26.2-fold lower than that of the wild-type strain on day 3 and 1.1-fold lower on day 7. The same sprays applied to both larvae and leaves for their feeding reduced the LC_(50) of the transformant 17.2- and 1.3-fold on days 3 and 7, respectively. Median lethal times (LT_(50)s) of BbV28 were shortened by 23 to 35%, declining with conidial concentrations. The larvae infected by ingestion of BbV28 conidia showed typical symptoms of Vip3A action, i.e., shrinkage and palsy. However, neither LC_(50) nor LT_(50) trends differed between BbV28 and its parental strain if the infection occurred through the cuticle only. Our findings indicate that fungal conidia can be used as vectors for spreading the highly insecticidal Vip3A protein for control of foliage feeders such as S. litura .
机译：昆虫病原性球孢白僵菌通过角质层感染缓慢作用于害虫。苏云金芽孢杆菌的营养杀虫蛋白（Vip3A）通过经口感染迅速杀死鳞翅目害虫，但其在害虫控制中的用途仅限于整合到转基因植物中。因此，创建了表达Vip3Aa1（Vip3A毒素）的转基因球孢杆菌菌株（BbV28），通过分生孢子的食入和角质层感染来感染东方菜蛾蛾斜纹夜蛾的幼虫。 Vip3Aa1（〜88 kDa）在BbV28的分生孢子胞质中高表达，并在分生孢子后18和36 h以幼虫中肠的消化形式（〜62 kDa）被检测到。 BbV28对以分生孢子悬浮液喷洒的卷心菜叶为食的第二龄幼虫的致死浓度（LC_（50））比第3天的野生型菌株低26.2倍，比第7天的低1.1倍对幼虫和叶片施用相同的喷雾剂以使其进食，分别在第3天和第7天将转化体的LC_（50）降低了17.2和1.3倍。 BbV28的中位致死时间（LT_（50）s）缩短了23％至35％，随分生孢子浓度的降低而降低。摄入BbV28分生孢子感染的幼虫表现出Vip3A作用的典型症状，即萎缩和麻痹。但是，如果仅通过角质层感染，则BbV28及其亲本菌株之间的LC_（50）和LT_（50）趋势均无差异。我们的研究结果表明，真菌分生孢子可以用作传播高度杀虫性Vip3A蛋白的载体，以控制树叶饲养者（如斜纹夜蛾）。

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