Microorganisms with Novel Dissimilatory (Bi)Sulfite Reductase Genes Are Widespread and Part of the Core Microbiota in Low-Sulfate Peatlands

Doris Steger; Cecilia Wentrup; Christina Braunegger; Pinsurang Deevong; Manuel Hofer; Andreas Richter; Christian Baranyi; Michael Pester; Michael Wagner; Alexander Loy

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Microorganisms with Novel Dissimilatory (Bi)Sulfite Reductase Genes Are Widespread and Part of the Core Microbiota in Low-Sulfate Peatlands

机译：具有新型异化（Bi）亚硫酸盐还原酶基因的微生物在低硫酸盐泥炭地广泛传播并且是部分核心微生物群的一部分。

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Peatlands of the Lehstenbach catchment (Germany) house as-yet-unidentified microorganisms with phylogenetically novel variants of the dissimilatory (bi)sulfite reductase genes dsrAB . These genes are characteristic of microorganisms that reduce sulfate, sulfite, or some organosulfonates for energy conservation but can also be present in anaerobic syntrophs. However, nothing is currently known regarding the abundance, community dynamics, and biogeography of these dsrAB -carrying microorganisms in peatlands. To tackle these issues, soils from a Lehstenbach catchment site (Schl?ppnerbrunnen II fen) from different depths were sampled at three time points over a 6-year period to analyze the diversity and distribution of dsrAB -containing microorganisms by a newly developed functional gene microarray and quantitative PCR assays. Members of novel, uncultivated dsrAB lineages (approximately representing species-level groups) (i) dominated a temporally stable but spatially structured dsrAB community and (ii) represented “core” members (up to 1% to 1.7% relative abundance) of the autochthonous microbial community in this fen. In addition, denaturing gradient gel electrophoresis (DGGE)- and clone library-based comparisons of the dsrAB diversity in soils from a wet meadow, three bogs, and five fens of various geographic locations (distance of ～1 to 400 km) identified that one Syntrophobacter -related and nine novel dsrAB lineages are widespread in low-sulfate peatlands. Signatures of biogeography in dsrB -based DGGE data were not correlated with geographic distance but could be explained largely by soil pH and wetland type, implying that the distribution of dsrAB -carrying microorganisms in wetlands on the scale of a few hundred kilometers is not limited by dispersal but determined by local environmental conditions.

机译：Lehstenbach流域（德国）的泥炭地拥有尚未鉴定的微生物，具有异化（亚）亚硫酸盐还原酶基因dsrAB的系统发育新变异。这些基因是减少硫酸盐，亚硫酸盐或某些有机磺酸盐以节约能源的微生物的特征，但也可以存在于厌氧菌中。但是，目前对于泥炭地中这些dsrAB携带微生物的丰度，群落动态和生物地理学还一无所知。为了解决这些问题，在6年的三个时间点对来自Lehstenbach集水区（Schl？ppnerbrunnen II fen）不同深度的土壤进行了采样，以通过一个新开发的功能基因分析含dsrAB的微生物的多样性和分布芯片和定量PCR分析。新型，未经培养的dsrAB世系成员（大约代表物种水平的群体）（i）占据了时间稳定但空间结构化的dsrAB群落的主体，并且（ii）代表了本地的“核心”成员（相对丰度高达1％至1.7％）在这个微生物群落中。此外，基于变性梯度凝胶电泳（DGGE）和克隆文库的方法比较了湿地，三个沼泽和五个不同地理位置（距离约1至400 km）的土壤中dsrAB的多样性，发现其中一个在低硫酸盐泥炭地中广泛存在与风化细菌相关的9种新的dsrAB谱系。基于dsrB的DGGE数据中的生物地理特征与地理距离无关，但可以很大程度上由土壤pH值和湿地类型来解释，这意味着在几百公里范围内湿地中携带dsrAB的微生物的分布不受限于分散，但取决于当地环境条件。

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《Applied Microbiology》 |2011年第4期|共12页
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Doris Steger; Cecilia Wentrup; Christina Braunegger; Pinsurang Deevong; Manuel Hofer; Andreas Richter; Christian Baranyi; Michael Pester; Michael Wagner; Alexander Loy;
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1. Microorganisms with Novel Dissimilatory (Bi)Sulfite Reductase Genes Are Widespread and Part of the Core Microbiota in Low-Sulfate Peatlands [J] . Doris Steger, Cecilia Wentrup, Christina Braunegger, Applied and Environmental Microbiology . 2011,第4期

机译：具有新型异化（Bi）亚硫酸盐还原酶基因的微生物在低硫酸盐泥炭地广泛传播并且是部分核心微生物群的一部分。
2. Microorganisms with Novel Dissimilatory (Bi)Sulfite Reductase Genes Are Widespread and Part of the Core Microbiota in Low-Sulfate Peatlands [J] . Doris Steger, Cecilia Wentrup, Christina Braunegger, Applied and Environmental Microbiology . 2011,第4期

机译：具有新型异化（Bi）亚硫酸盐还原酶基因的微生物在低硫酸盐泥炭地广泛传播并且是部分核心微生物群的一部分。
3. Microorganisms with Novel Dissimilatory (Bi)Sulfite Reductase Genes Are Widespread and Part of the Core Microbiota in Low-Sulfate Peatlands [J] . Doris Steger, Cecilia Wentrup, Christina Braunegger, Applied Microbiology . 2011,第4期

机译：具有新型异化（Bi）亚硫酸盐还原酶基因的微生物在低硫酸盐泥炭地广泛传播并且是部分核心微生物群的一部分。
4. Detection of SRP Activity by Quantification of mRNA for the Dissimilatory (bi) Sulfite Reductase Gene (dsrA) by Reverse Transcription Quantitative PCR [C] . Andy Price, Laura Acuna Alvarez, Corinne Whitby, Corrosion conference and expo 2010 . 2010

机译：通过反转录定量PCR检测异源（bi）亚硫酸还原酶基因（dsrA）的mRNA定量检测SRP活性
5. Molecular characterization of sulfidogenic communities among pristine and polluted sites based on the dissimilatory sulfite reductase genes (DSRAB). [D] . Perez-Jimenez, Jose R. 2003

机译：基于异化亚硫酸盐还原酶基因（DSRAB）的原始和污染位点之间的生硫群落的分子特征。
6. Microorganisms with Novel Dissimilatory (Bi)Sulfite Reductase Genes Are Widespread and Part of the Core Microbiota in Low-Sulfate Peatlands [O] . Doris Steger, Cecilia Wentrup, Christina Braunegger, 2011

机译：具有新型异化（Bi）亚硫酸盐还原酶基因的微生物在低硫酸盐泥炭地广泛传播并且是部分核心微生物群的一部分。
7. Microorganisms with Novel Dissimilatory (Bi)Sulfite Reductase Genes Are Widespread and Part of the Core Microbiota in Low-Sulfate Peatlands [O] . Steger, Doris, University of Vienna, Wentrup, Cecilia, 2011

机译：具有新型异化（Bi）亚硫酸盐还原酶基因的微生物在低硫酸盐泥炭地广泛传播并且是部分核心微生物群的一部分。
8. Field calibration of soil-core microcosms for evaluating fate and effects of genetically engineered microorganisms in terrestrial ecosystems. [R] . H. Bolton J. K. Fredrickson S. A. Bentjen D. J. Workman S. W. Li 1991

机译：土壤核心微观世界的现场标定，用于评估陆地生态系统中基因工程微生物的命运和影响。

Microorganisms with Novel Dissimilatory (Bi)Sulfite Reductase Genes Are Widespread and Part of the Core Microbiota in Low-Sulfate Peatlands

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