...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Total and Viable Legionella pneumophila Cells in Hot and Natural Waters as Measured by Immunofluorescence-Based Assays and Solid-Phase Cytometry
【24h】

Total and Viable Legionella pneumophila Cells in Hot and Natural Waters as Measured by Immunofluorescence-Based Assays and Solid-Phase Cytometry

机译：通过基于免疫荧光的分析和固相细胞仪测定的热水和天然水中的嗜肺军团菌总细胞和存活细胞

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A new method was developed for the rapid and sensitive detection of viable Legionella pneumophila . The method combines specific immunofluorescence (IF) staining using monoclonal antibodies with a bacterial viability marker (ChemChrome V6 cellular esterase activity marker) by means of solid-phase cytometry (SPC). IF methods were applied to the detection and enumeration of both the total and viable L. pneumophila cells in water samples. The sensitivity of the IF methods coupled to SPC was 34 cells liter~(?1), and the reproducibility was good, with the coefficient of variation generally falling below 30%. IF methods were applied to the enumeration of total and viable L. pneumophila cells in 46 domestic hot water samples as well as in cooling tower water and natural water samples, such as thermal spring water and freshwater samples. Comparison with standard plate counts showed that (i) the total direct counts were always higher than the plate counts and (ii) the viable counts were higher than or close to the plate counts. With domestic hot waters, when the IF assay was combined with the viability test, SPC detected up to 3.4 × 10~(3) viable but nonculturable L. pneumophila cells per liter. These direct IF methods could be a powerful tool for high-frequency monitoring of domestic hot waters or for investigating the occurrence of viable L. pneumophila in both man-made water systems and environmental water samples.
机译：开发了一种快速，灵敏的活军团菌检测方法。该方法结合了使用单克隆抗体的特异性免疫荧光（IF）染色和细菌生存力标记（ChemChrome V6细胞酯酶活性标记），并通过固相细胞计数（SPC）进行了结合。 IF方法用于检测和计数水样中总的和存活的嗜肺乳杆菌细胞。 IF法与SPC结合的灵敏度为34细胞升〜（？1），重现性好，变异系数一般低于30％。 IF方法应用于46个生活热水样品以及冷却塔水和天然水样品（如温泉水和淡水样品）中总的和存活的嗜肺乳杆菌细胞的计数。与标准板数的比较显示（i）总直接数始终高于板数，并且（ii）可行数高于或接近板数。在生活热水中，将IF分析与可行性测试结合使用时，SPC每升最多可检测到3.4×10〜（3）个可存活但不可培养的嗜肺乳杆菌细胞。这些直接中频方法可能是用于高频监测生活热水或调查人造水系统和环境水样中是否存在嗜肺乳杆菌的强大工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号