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首页> 外文期刊>Applied Microbiology >Characterization of Three Lactic Acid Bacteria and Their Isogenic ldh Deletion Mutants Shows Optimization for YATP (Cell Mass Produced per Mole of ATP) at Their Physiological pHs
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Characterization of Three Lactic Acid Bacteria and Their Isogenic ldh Deletion Mutants Shows Optimization for YATP (Cell Mass Produced per Mole of ATP) at Their Physiological pHs

机译：三种乳酸菌及其等价ldh缺失突变体的表征显示YATP（每摩尔ATP产生的细胞质量）在其生理pH下的优化

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Several lactic acid bacteria use homolactic acid fermentation for generation of ATP. Here we studied the role of the lactate dehydrogenase enzyme on the general physiology of the three homolactic acid bacteria Lactococcus lactis , Enterococcus faecalis , and Streptococcus pyogenes . Of note, deletion of the ldh genes hardly affected the growth rate in chemically defined medium under microaerophilic conditions. However, the growth rate was affected in rich medium. Furthermore, deletion of ldh affected the ability for utilization of various substrates as a carbon source. A switch to mixed acid fermentation was observed during glucose-limited continuous growth and was dependent on the growth rate for S. pyogenes and on the pH for E. faecalis . In S. pyogenes and L. lactis , a change in pH resulted in a clear change in Y _(ATP) (cell mass produced per mole of ATP). The pH that showed the highest Y _(ATP) corresponded to the pH of the natural habitat of the organisms.
机译：几种乳酸菌使用高乳酸发酵产生ATP。在这里，我们研究了乳酸脱氢酶对三种乳酸菌乳酸乳球菌，粪肠球菌和化脓性链球菌的一般生理作用。值得注意的是，ldh基因的缺失几乎不会影响微需氧条件下化学成分确定的培养基中的生长速率。但是，在丰富的培养基中，增长率受到影响。此外，ldh的缺失影响了利用各种底物作为碳源的能力。在葡萄糖有限的连续生长过程中，观察到向混合酸发酵的转换，并且取决于化脓性链球菌的生长速率和粪肠球菌的pH。在化脓性链球菌和乳酸乳球菌中，pH的变化导致Y_（ATP）（每摩尔ATP产生的细胞质量）发生明显变化。显示最高Y _（ATP）的pH值对应于生物自然栖息地的pH值。

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