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首页> 外文期刊>Applied Microbiology >Improved Eco-Friendly Recombinant Anabaena sp. Strain PCC7120 with Enhanced Nitrogen Biofertilizer Potential
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Improved Eco-Friendly Recombinant Anabaena sp. Strain PCC7120 with Enhanced Nitrogen Biofertilizer Potential

机译：改进的生态友好型鱼腥藻重组体。具有增强的氮生物肥料潜力的PCC7120菌株

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摘要

Photosynthetic, nitrogen-fixing Anabaena strains are native to tropical paddy fields and contribute to the carbon and nitrogen economy of such soils. Genetic engineering was employed to improve the nitrogen biofertilizer potential of Anabaena sp. strain PCC7120. Constitutive enhanced expression of an additional integrated copy of the hetR gene from a light-inducible promoter elevated HetR protein expression and enhanced functional heterocyst frequency in the recombinant strain. The recombinant strain displayed consistently higher nitrogenase activity than the wild-type strain and appeared to be in homeostasis with compatible modulation of photosynthesis and respiration. The enhanced combined nitrogen availability from the recombinant strain positively catered to the nitrogen demand of rice seedlings in short-term hydroponic experiments and supported better growth. The engineered strain is stable, eco-friendly, and useful for environmental application as nitrogen biofertilizer in paddy fields.
机译：光合作用，固氮的鱼腥藻菌株是热带稻田的原生品种，有助于此类土壤的碳氮经济。利用基因工程来提高鱼腥藻的氮生物肥料潜力。菌株PCC7120。来自光诱导型启动子的hetR基因的另一个整合拷贝的组成型增强表达提高了重组菌株中HetR蛋白的表达并增强了功能性异囊藻的频率。重组菌株始终显示出比野生型菌株更高的固氮酶活性，并且似乎处于与光合作用和呼吸作用相适应的稳态中。在短期水培试验中，重组菌株提高了组合氮的利用率，积极满足了水稻幼苗对氮的需求，并支持了更好的生长。该工程菌株稳定，环保，可作为稻田中的氮生物肥料用于环境应用。

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