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首页> 外文期刊>Applied Microbiology >Genotypic and Chemotypic Diversity of Neotyphodium Endophytes in Tall Fescue from Greece
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Genotypic and Chemotypic Diversity of Neotyphodium Endophytes in Tall Fescue from Greece

机译：希腊高羊茅中新伤寒内生菌的基因型和化学型多样性

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Epichloid endophytes provide protection from a variety of biotic and abiotic stresses for cool-season grasses, including tall fescue. A collection of 85 tall fescue lines from 15 locations in Greece, including both Continental and Mediterranean germplasm, was screened for the presence of native endophytes. A total of 37 endophyte-infected lines from 10 locations were identified, and the endophytes were classified into five distinct groups (G1 to G5) based on physical characteristics such as colony morphology, growth rate, and conidial morphology. These classifications were supported by phylogenetic analyses of housekeeping genes tefA and tubB , and the endophytes were further categorized as Neotyphodium coenophialum isolates (G1, G4, and G5) or Neotyphodium sp. FaTG-2 ( Festuca arundinacea taxonomic group 2 isolates (G2 and G3). Analyses of the tall fescue matK chloroplast genes indicated a population-wide, host-specific association between N. coenophialum and Continental tall fescue and between FaTG-2 and Mediterranean tall fescue that was also reflected by differences in colonization of host tillers by the native endophytes. Genotypic analyses of alkaloid gene loci combined with chemotypic (chemical phenotype) profiles provided insight into the genetic basis of chemotype diversity. Variation in alkaloid gene content, specifically the presence and absence of genes, and copy number of gene clusters explained the alkaloid diversity observed in the endophyte-infected tall fescue, with one exception. The results from this study provide insight into endophyte germplasm diversity present in living tall fescue populations.
机译：表皮内生菌能够保护凉季草（包括高羊茅草）免受各种生物和非生物胁迫。筛选了来自希腊15个地区的85个高羊茅线，包括大陆种和地中海种质，以寻找天然内生菌的存在。共鉴定了来自10个位置的37种内生细菌感染品系，并根据菌落形态，生长速率和分生孢子形态等物理特征将内生细菌分为五个不同的组（G1至G5）。这些分类得到管家基因tefA和tubB的系统发育分析的支持，并且将内生菌进一步分类为新鼠疫分离株（G1，G4和G5）或新鼠疫sp。 FaTG-2（大麦草分类学分类组2分离株（G2和G3）。对高羊茅matK叶绿体基因的分析表明，猪粪肠球菌和大陆高羊茅之间以及FaTG-2和地中海高羊茅之间具有群体范围的宿主特异性关联。羊茅还通过天然内生菌在分till定植中的差异反映出来，对生物碱基因位点的基因型分析与化学型（化学表型）相结合，为化学型多样性的遗传基础提供了见识。基因的缺失，缺乏以及基因簇的拷贝数解释了在被内生菌感染的高羊茅中观察到的生物碱多样性，但有一项例外，这项研究的结果为活着的高羊茅种群中存在的内生菌种质多样性提供了见识。

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