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首页> 外文期刊>Applied Microbiology >Multilocus Sequence Typing Scheme for the Characterization of 936-Like Phages Infecting Lactococcus lactis
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Multilocus Sequence Typing Scheme for the Characterization of 936-Like Phages Infecting Lactococcus lactis

机译：多基因座序列分型方案，用于表征感染乳酸乳球菌的936类噬菌体

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Lactococcus lactis phage infections are costly for the dairy industry because they can slow down the fermentation process and adversely impact product safety and quality. Although many strategies have been developed to better control phage populations, new virulent phages continue to emerge. Thus, it is beneficial to develop an efficient method for the routine identification of new phages within a dairy plant to rapidly adapt antiphage tactics. Here, we present a multilocus sequence typing (MLST) scheme for the characterization of the 936-like phages, the most prevalent phage group infecting L. lactis strains worldwide. The proposed MLST system targets the internal portion of five highly conserved genomic sequences belonging to the packaging, morphogenesis, and lysis modules. Our MLST scheme was used to analyze 100 phages with different restriction fragment length polymorphism (RFLP) patterns isolated from 11 different countries between 1971 and 2010. PCR products were obtained for all the phages analyzed, and sequence analysis highlighted the high discriminatory power of the MLST system, detecting 93 different sequence types. A conserved locus within the lys gene (coding for endolysin) was the most discriminative, with 65 distinct alleles. The locus within the mcp gene (major capsid protein) was the most conserved (54 distinct alleles). Phylogenetic analyses of the concatenated sequences exhibited a strong concordance of the clusters with the phage host range, indicating the clonal evolution of these phages. A public database has been set up for the proposed MLST system, and it can be accessed at http://pubmlst.org/bacteriophages/.
机译：乳酸乳球菌噬菌体感染对于乳制品行业而言是昂贵的，因为它们会减慢发酵过程并不利地影响产品的安全性和质量。尽管已开发出许多策略来更好地控制噬菌体种群，但新的有毒噬菌体仍在不断涌现。因此，有益的是开发一种用于常规鉴定乳品厂中的新噬菌体以快速适应抗噬菌体策略的有效方法。在这里，我们提出了一种多基因座序列分型（MLST）方案，用于表征936样噬菌体，这是全世界感染乳酸杆菌的最普遍的噬菌体群。拟议的MLST系统针对五个高度保守的基因组序列的内部，这些序列属于包装，形态发生和裂解模块。我们的MLST方案用于分析1971年至2010年间从11个不同国家分离的100个具有不同限制性片段长度多态性（RFLP）模式的噬菌体。获得了所有被分析噬菌体的PCR产物，并且序列分析突出了MLST的高识别力系统，检测93种不同的序列类型。 lys基因（编码内溶素）中的保守位点是最有区别的，具有65个不同的等位基因。 mcp基因（主要衣壳蛋白）中的基因座是最保守的（54个不同的等位基因）。串联序列的系统发生分析显示簇与噬菌体宿主范围的高度一致性，表明这些噬菌体的克隆进化。已为拟议的MLST系统建立了一个公共数据库，可以从http://pubmlst.org/bacteriophages/进行访问。

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