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首页> 外文期刊>Applied Microbiology >Responses of Methanogen mcrA Genes and Their Transcripts to an Alternate Dry/Wet Cycle of Paddy Field Soil
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Responses of Methanogen mcrA Genes and Their Transcripts to an Alternate Dry/Wet Cycle of Paddy Field Soil

机译：产甲烷菌mcrA基因及其转录本对稻田土壤干湿交替变化的响应

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Intermittent drainage can substantially reduce methane emission from rice fields, but the microbial mechanisms remain poorly understood. In the present study, we determined the rates of methane production and emission, the dynamics of ferric iron and sulfate, and the abundance of methanogen mcrA genes (encoding the alpha subunit of methyl coenzyme M reductase) and their transcripts in response to alternate dry/wet cycles in paddy field soil. We found that intermittent drainage did not affect the growth of rice plants but significantly reduced the rates of both methane production and emission. The dry/wet cycles also resulted in shifts of soil redox conditions, increasing the concentrations of ferric iron and sulfate in the soil. Quantitative PCR analysis revealed that both mcrA gene copies and mcrA transcripts significantly decreased after dry/wet alternation compared to continuous flooding. Correlation and regression analyses showed that the abundance of mcrA genes and transcripts positively correlated with methane production potential and soil water content and negatively correlated with the concentrations of ferric iron and sulfate in the soil. However, the transcription of mcrA genes was reduced to a greater extent than the abundance of mcrA genes, resulting in very low mcrA transcript/gene ratios after intermittent drainage. Furthermore, terminal restriction fragment length polymorphism analysis revealed that the composition of methanogenic community remained stable under dry/wet cycles, whereas that of metabolically active methanogens strongly changed. Collectively, our study demonstrated a stronger effect of intermittent drainage on the abundance of mcrA transcripts than of mcrA genes in rice field soil.
机译：间歇排水可以大大减少稻田中的甲烷排放，但是微生物机理仍然知之甚少。在本研究中，我们确定了甲烷产生和排放的速率，三价铁和硫酸铁的动力学以及产甲烷菌mcrA基因（编码甲基辅酶M还原酶的α亚基）的丰度及其转录物对干/湿交替的响应。稻田土壤的湿循环。我们发现间歇性排水不会影响水稻植株的生长，但会显着降低甲烷的产生和排放速率。干/湿循环还导致土壤氧化还原条件的改变，增加了土壤中铁和硫酸铁的浓度。定量PCR分析显示，与连续淹水相比，干/湿交替后mcrA基因拷贝和mcrA转录物均显着降低。相关和回归分析表明，mcrA基因和转录本的丰度与甲烷生产潜力和土壤含水量呈正相关，与土壤中三价铁和硫酸盐的含量呈负相关。但是，与丰富的mcrA基因相比，mcrA基因的转录降低程度更大，导致间歇性引流后mcrA转录物/基因比率非常低。此外，末端限制性片段长度多态性分析显示，产甲烷菌群落的组成在干/湿循环下保持稳定，而代谢活性产甲烷菌的组成发生了强烈变化。总体而言，我们的研究表明，在稻田土壤中，间歇性排水对mcrA转录物的丰度比mcrA基因的丰度强。

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