Direct Assessment of Metabolite Utilization by Pseudomonas aeruginosa during Growth on Artificial Sputum Medium

Volker Behrends; Benedikt Geier; Huw D. Williams; Jacob G. Bundy

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Direct Assessment of Metabolite Utilization by Pseudomonas aeruginosa during Growth on Artificial Sputum Medium

机译：人工痰培养基中铜绿假单胞菌生长过程中代谢物利用的直接评估

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We grew Pseudomonas aeruginosa in LB and artificial sputum medium (ASM) (filtered and unfiltered) and quantified metabolite utilization and excretion by nuclear magnetic resonance (NMR) spectroscopy (metabolic footprinting or extracellular metabolomics). Utilization rates were similar between media, but there were differences in excretion—e.g., acetate was produced only in unfiltered ASM.

机译：我们在LB和人工痰培养基（ASM）（过滤和未过滤）中培养了铜绿假单胞菌，并通过核磁共振（NMR）光谱（代谢足迹或细胞外代谢组学）定量了代谢物的利用和排泄。培养基之间的利用率相似，但排泄率存在差异，例如，乙酸盐仅在未过滤的ASM中产生。

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《Applied Microbiology》 |2013年第7期|共4页
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Volker Behrends; Benedikt Geier; Huw D. Williams; Jacob G. Bundy;
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1. Direct Assessment of Metabolite Utilization by Pseudomonas aeruginosa during Growth on Artificial Sputum Medium [J] . Volker Behrends, Benedikt Geier, Huw D. Williams, Applied and Environmental Microbiology . 2013,第7期

机译：人工痰培养基中铜绿假单胞菌生长过程中代谢物利用的直接评估
2. Phenotypical Characteristics of the Black Yeast Exophiala dermatitidis Are Affected by Pseudomonas aeruginosa in an Artificial Sputum Medium Mimicking Cystic Fibrosis–Like Conditions [J] . Lisa Kirchhoff, Ann-Kathrin Weisner, Mona Schrepffer, Frontiers in Microbiology . 2020,第6期

机译：黑色酵母的表型特征<斜斜体> exophiala dermatitidis 在人工痰介质中的 pseudomonas erginosa 模仿囊性纤维化的状况
3. Sub-inhibitory concentrations of some antibiotics can drive diversification of Pseudomonas aeruginosa populations in artificial sputum medium [J] . Elli A Wright, Joanne L Fothergill, Steve Paterson, BMC Microbiology . 2013,第1期

机译：一些抗生素的副抑制浓度可以在人造痰介质中驱动假单胞菌铜绿假单胞菌种群的多样化
4. Electrochemistry provides a simple way to monitor Pseudomonas aeruginosa metabolites [C] . Oziat Julie, Elsen Sylvie, Owens Roisin M., Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2015

机译：电化学提供了一种简单的方法来监测铜绿假单胞菌的代谢产物
5. Role of Respiratory Enzymes on Growth of Pseudomonas aeruginosa in Luria-Bertani and Artificial Urine Media [D] . Hu, Yuyao. 2018

机译：呼吸酶对人工尿液中假单胞菌铜绿假单胞菌生长的作用
6. Direct Assessment of Metabolite Utilization by Pseudomonas aeruginosa during Growth on Artificial Sputum Medium [O] . Volker Behrends, Benedikt Geier, Huw D. Williams, 2013

机译：人工痰培养基中铜绿假单胞菌生长过程中代谢物利用的直接评估
7. Commercial porcine gastric mucin contributes to variation in production of small molecule virulence factors by Pseudomonas aeruginosa when cultured in different formulations of artificial sputum medium [O] . Rachel L. Neve, Brent D. Carrillo, Vanessa V. Phelan 2021

机译：商业猪胃粘蛋白有助于在人造痰介质的不同配方中培养时，铜绿假单胞菌生产小分子毒力因子的变化

Direct Assessment of Metabolite Utilization by Pseudomonas aeruginosa during Growth on Artificial Sputum Medium

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