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首页> 外文期刊>Applied Microbiology >Environmental Factors Influencing the Prevalence of a Clostridium botulinum Type C/D Mosaic Strain in Nonpermanent Mediterranean Wetlands
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Environmental Factors Influencing the Prevalence of a Clostridium botulinum Type C/D Mosaic Strain in Nonpermanent Mediterranean Wetlands

机译:影响非永久性地中海湿地肉毒梭状芽孢杆菌C / D型马赛克菌株流行的环境因素

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Between 1978 and 2008, 13 avian botulism outbreaks were recorded in the wetlands of Mancha Húmeda (central Spain). These outbreaks caused the deaths of around 20,000 birds from over 50 species, including globally endangered white-headed ducks ( Oxyura leucoceophala ). Here, a significant association was found between the number of dead birds recorded in each botulism outbreak and the mean temperature in July (always >26°C). The presence of Clostridium botulinum type C/D in wetland sediments was detected by real-time PCR (quantitative PCR [qPCR]) in 5.8% of 207 samples collected between 2005 and 2008. Low concentrations of Cl~(?) and high organic matter content in sediments were significantly associated with the presence of C. botulinum . Seventy-five digestive tracts of birds found dead during botulism outbreaks were analyzed; C. botulinum was present in 38.7% of them. The prevalence of C. botulinum was 18.2% ( n = 22 pools) in aquatic invertebrates (Chironomidae and Corixidae families) and 33.3% ( n = 18 pools) in necrophagous invertebrates (Sarcophagidae and Calliphoridae families), including two pools of adult necrophagous flies collected around bird carcasses. The presence of the bacteria in the adult fly form opens up new perspectives in the epidemiology of avian botulism, since these flies may be transporting C. botulinum from one carcass to another.
机译:1978年至2008年之间,在ManchaHúmeda(西班牙中部)的湿地中记录到13次禽肉毒杆菌暴爆发。这些暴发导致50多种物种的大约20,000只鸟死亡,其中包括全球濒危的白头鸭(Oxyura leucoceophala)。在这里,发现在每次肉毒杆菌中毒爆发中记录的死亡禽鸟数量与七月的平均温度(始终> 26°C)之间存在显着关联。通过实时PCR(定量PCR [qPCR]),在2005年至2008年间收集的207个样本中,有5.8%检测到了C / D型肉毒梭菌的存在。低浓度的Cl〜(?)和高有机质沉积物的含量与肉毒梭菌的存在密切相关。分析了肉毒中毒爆发期间发现死鸟的75消化道;其中38.7%的肉毒梭菌存在。在水生无脊椎动物(Chironomidae和Corixidae家族)中,肉毒梭菌的患病率为18.2%(n = 22个池),在食肉性无脊椎动物(Sarcophagidae和Calliphoridae家庭)中,包括两个成年的食虫性蝇蝇,其发生率为33.2%(n = 18个池)。收集在鸟的尸体周围。成年果蝇中细菌的存在为禽肉毒杆菌病的流行病学开辟了新的视角,因为这些果蝇可能会将肉毒梭菌从一个car体转移到另一个car体。

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