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首页> 外文期刊>Applied Microbiology >Interspecific Quorum Sensing Mediates the Resuscitation of Viable but Nonculturable Vibrios
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Interspecific Quorum Sensing Mediates the Resuscitation of Viable but Nonculturable Vibrios

机译：种间群体感应介导存活但不可培养的弧菌的复苏。

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摘要

Entry and exit from dormancy are essential survival mechanisms utilized by microorganisms to cope with harsh environments. Many bacteria, including the opportunistic human pathogen Vibrio vulnificus , enter a form of dormancy known as the viable but nonculturable (VBNC) state. VBNC cells can resuscitate when suitable conditions arise, yet the molecular mechanisms facilitating resuscitation in most bacteria are not well understood. We discovered that bacterial cell-free supernatants (CFS) can awaken preexisting dormant vibrio populations within oysters and seawater, while CFS from a quorum sensing mutant was unable to produce the same resuscitative effect. Furthermore, the quorum sensing autoinducer AI-2 could induce resuscitation of VBNC V. vulnificus in vitro , and VBNC cells of a mutant unable to produce AI-2 were unable to resuscitate unless the cultures were supplemented with exogenous AI-2. The quorum sensing inhibitor cinnamaldehyde delayed the resuscitation of wild-type VBNC cells, confirming the importance of quorum sensing in resuscitation. By monitoring AI-2 production by VBNC cultures over time, we found quorum sensing signaling to be critical for the natural resuscitation process. This study provides new insights into the molecular mechanisms stimulating VBNC cell exit from dormancy, which has significant implications for microbial ecology and public health.
机译：休眠的进入和退出是微生物用来应对恶劣环境的基本生存机制。许多细菌，包括机会性人类病原体弧菌，都进入了一种休眠形式，称为存活但不可培养（VBNC）状态。当合适的条件出现时，VBNC细胞可以复苏，但是大多数细菌中促进复苏的分子机制尚不十分清楚。我们发现无细菌的上清液（CFS）可以唤醒牡蛎和海水中预先存在的休眠弧菌种群，而来自群体感应突变体的CFS无法产生相同的复苏作用。此外，群体感应自动诱导物AI-2可以在体外诱导VBNC V. vulnificus的复苏，除非培养物中添加了外源AI-2，否则无法产生AI-2的突变体的VBNC细胞无法复苏。群体感应抑制剂肉桂醛延迟了野生型VBNC细胞的复苏，证实了群体感应在复苏中的重要性。通过长时间监控VBNC培养的AI-2产量，我们发现群体感应信号对于自然复苏过程至关重要。这项研究提供了新的见解，激发了VBNC细胞从休眠状态退出的分子机制，这对微生物生态学和公共健康具有重要意义。

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