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首页> 外文期刊>Applied Microbiology >A Rice Gene for Microbial Symbiosis, Oryza sativaCCaMK, Reduces CH4 Flux in a Paddy Field with Low Nitrogen Input
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A Rice Gene for Microbial Symbiosis, Oryza sativaCCaMK, Reduces CH4 Flux in a Paddy Field with Low Nitrogen Input

机译：水稻共生水稻基因Oryza sativaCCaMK降低了低氮输入的稻田中的CH4通量

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摘要

Plants have mutualistic symbiotic relationships with rhizobia and fungi by the common symbiosis pathway, of which Ca~(2+)/calmodulin-dependent protein kinase (encoded by CCaMK ) is a central component. Although Oryza sativa CCaMK ( OsCCaMK ) is required for fungal accommodation in rice roots, little is known about the role of OsCCaMK in rice symbiosis with bacteria. Here, we report the effect of a Tos17 -induced OsCCaMK mutant (NE1115) on CH_(4) flux in low-nitrogen (LN) and standard-nitrogen (SN) paddy fields compared with wild-type (WT) Nipponbare. The growth of NE1115 was significantly decreased compared with that of the WT, especially in the LN field. The CH_(4) flux of NE1115 in the LN field was significantly greater (156 to 407% in 2011 and 170 to 816% in 2012) than that of the WT, although no difference was observed in the SN field. The copy number of pmoA (encodes methane monooxygenase in methanotrophs) was significantly higher in the roots and rhizosphere soil of the WT than in those of NE1115. However, the mcrA (encodes methyl coenzyme M reductase in methanogens) copy number did not differ between the WT and NE1115. These results were supported by a ~(13)C-labeled CH_(4)-feeding experiment. In addition, the natural abundance of ~(15)N in WT shoots (3.05‰) was significantly lower than in NE1115 shoots (3.45‰), suggesting greater N_(2) fixation in the WT because of dilution with atmospheric N_(2) (0.00‰). Thus, CH_(4) oxidation and N_(2) fixation were simultaneously activated in the root zone of WT rice in the LN field and both processes are likely controlled by OsCCaMK .
机译：植物通过共同的共生途径与根瘤菌和真菌具有共生共生关系，其中Ca〜（2 +）/钙调蛋白依赖性蛋白激酶（由CCaMK编码）是其中的重要组成部分。尽管水稻根部的真菌适应需要水稻（Oryza sativa）CCaMK（OsCCaMK），但对OsCCaMK在水稻与细菌共生中的作用知之甚少。在这里，我们报告了Tos17诱导的OsCCaMK突变体（NE1115）对低氮（LN）和标准氮（SN）稻田中的CH_（4）通量的影响，与野生型（WT）日本晴相比。与WT相比，NE1115的生长显着下降，尤其是在LN领域。 LN场中NE1115的CH_（4）通量比WT大得多（2011年为156至407％，2012年为170至816％），尽管在SN场中未观察到差异。在WT的根和根际土壤中，pmoA的拷贝数（在甲烷氧化菌中编码甲烷单加氧酶）明显高于NE1115。但是，mcrA（在产甲烷菌中编码甲基辅酶M还原酶）的拷贝数在WT和NE1115之间没有差异。这些结果得到〜（13）C标记的CH_（4）-进料实验的支持。此外，WT芽中〜（15）N的自然丰度（3.05‰）明显低于NE1115芽中的（3.45‰），这表明由于大气中N_（2）的稀释，WT中的N_（2）固色更大。 （0.00‰）。因此，CH_（4）的氧化作用和N_（2）的固定作用在LN田的野生稻根部区域同时被激活，这两个过程都可能受OsCCaMK的控制。

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