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首页> 外文期刊>Applied Microbiology >Influence of prgH on the Persistence of Ingested Salmonella enterica in the Leafhopper Macrosteles quadrilineatus
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Influence of prgH on the Persistence of Ingested Salmonella enterica in the Leafhopper Macrosteles quadrilineatus

机译：prgH对叶蝉大叶蝗中消化小肠沙门氏菌持久性的影响

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摘要

Phytophagous insects can encounter Salmonella enterica on contaminated plant surfaces and transmit externally adhered and internalized bacteria on and among leaves. Excretion of ingested S. enterica by the leafhopper Macrosteles quadrilineatus has been previously reported; however, the sites of persistence of ingested bacteria remain undetermined. Fluorescence microscopy revealed the presence and persistence of S. enterica in various organs of M. quadrilineatus fed an inoculated diet for 12 h and then moved to two consecutive noninoculated diets for a total of 48 h. Ingested S. enterica was predominantly observed in the filter chamber, midgut, and Malpighian tubules of M. quadrilineatus dissected immediately after acquisition and at 24- and 48-h post-acquisition access periods (post-AAPs). Additionally, we examined the potential roles of the Salmonella pathogenicity island 1 (SPI-1) and SPI-2 type III secretion systems (T3SSs) in the persistence and excretion of ingested S. enterica . In competition assays, a prgH mutant lacking a functional SPI-1 T3SS was recovered at significantly lower levels than the WT in insect homogenates at 24 h post-AAP, and complementation with prgH restored S. enterica persistence in M. quadrilineatus . Moreover, expression of prgH inside M. quadrilineatus was observed up to 48 post-AAP. No differences were observed between the WT and an ssaK mutant lacking a functional SPI-2 T3SS in insect homogenates or between the WT and either mutant in insect excretions. This study provides novel insight into the presence and persistence of S. enterica inside M. quadrilineatus and demonstrates that the SPI-1 T3SS influences the persistence of the pathogen in the gut of a potential vector.
机译：食草昆虫可在受污染的植物表面遇到肠道沙门氏菌，并在叶片上和叶片间传播外部粘附和内在化的细菌。以前有报道说叶蝉Macrosteles quadrilineatus会排泄摄入的链球菌。但是，摄入细菌的持久性部位仍未确定。荧光显微镜检查显示，在饲喂接种饮食12 h的四支线虫的各个器官中，肠炎链球菌的存在和持续存在，然后转移到两个连续的未接种饮食中，共48 h。主要在采集后以及采集后24和48小时内（AAP后）切开四分枝杆菌的滤房，中肠和马尔皮安小管中观察到摄入的链球菌。此外，我们检查了沙门氏菌致病岛1（SPI-1）和SPI-2 III型分泌系统（T3SSs）在摄入的链球菌持续性和排泄中的潜在作用。在竞争试验中，在AAP后24小时内，昆虫匀浆中的prgH突变体缺乏功能性SPI-1 T3SS的回收率显着低于野生型，并且与prgH的互补可恢复四叉线虫的肠道链球菌持久性。此外，直到AAP后48小时，观察到四分枝杆菌内prgH的表达。在昆虫匀浆中，WT与缺少功能性SPI-2 T3SS的ssaK突变体之间，或者在WT与昆虫排泄物中的任一突变体之间，未观察到差异。这项研究提供了新的见解，在四线虫内肠球菌的存在和持久性，并证明SPI-1 T3SS影响病原体在潜在载体的肠道中的持久性。

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