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首页> 外文期刊>Applied Microbiology >Methanotrophic and Methanogenic Communities in Swiss Alpine Fens Dominated by Carex rostrata and Eriophorum angustifolium
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Methanotrophic and Methanogenic Communities in Swiss Alpine Fens Dominated by Carex rostrata and Eriophorum angustifolium

机译:由Carex rostrata和Eriophorum angustifolium占主导的瑞士高山芬中的甲烷营养和产甲烷菌群落

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Vascular plants play a key role in controlling CH_(4) emissions from natural wetlands, because they influence CH_(4) production, oxidation, and transport to the atmosphere. Here we investigated differences in the abundance and composition of methanotrophic and methanogenic communities in three Swiss alpine fens dominated by different vascular plant species under natural conditions. The sampling locations either were situated at geographically distinct sites with different physicochemical properties but the same dominant plant species ( Carex rostrata ) or were located within the same site, showing comparable physicochemical pore water properties, but had different plant species ( C. rostrata or Eriophorum angustifolium ). All three locations were permanently submerged and showed high levels of CH_(4) emissions (80.3 to 184.4 mg CH_(4) m~(?2) day~(?1)). Soil samples were collected from three different depths with different pore water CH_(4) and O_(2) concentrations and were analyzed for pmoA and mcrA gene and transcript abundance and community composition, as well as soil structure. The dominant plant species appeared to have a significant influence on the composition of the active methanotrophic communities (transcript level), while the methanogenic communities differed significantly only at the gene level. Yet no plant species-specific microbial taxa were discerned. Moreover, for all communities, differences in composition were more pronounced with the site (i.e., with different physicochemical properties) than with the plant species. Moreover, depth significantly influenced the composition of the active methanotrophic communities. Differences in abundance were generally low, and active methanotrophs and methanogens coexisted at all three locations and depths independently of CH_(4) and O_(2) concentrations or plant species.
机译:血管植物在控制天然湿地CH_(4)的排放中起着关键作用,因为它们影响CH_(4)的生产,氧化和向大气的运输。在这里,我们调查了在自然条件下由不同维管植物物种支配的三个瑞士高山中甲烷营养和产甲烷群落的丰度和组成差异。采样地点位于具有不同理化特性但具有相同优势植物物种(Carex rostrata)的地理上不同的地点,或位于同一地点,显示出可比较的理化孔隙水特性,但是具有不同的植物物种(C. rostrata或Eriophorum) Angustifolium)。这三个地点均被永久淹没,并显示出高水平的CH_(4)排放(80.3至184.4 mg CH_(4)m〜(?2)天〜(?1))。从三个不同深度,不同孔隙水CH_(4)和O_(2)浓度收集土壤样品,并分析其pmoA和mcrA基因,转录本丰度和群落组成以及土壤结构。优势植物物种似乎对活性甲烷营养群落的组成(转录本水平)具有重要影响,而产甲烷菌群落仅在基因水平上有显着差异。然而,没有发现特定于植物物种的微生物分类群。而且,对于所有社区而言,组成部位的差异(即具有不同的理化性质)比植物物种更为明显。此外,深度显着影响了活性甲烷营养型群落的组成。丰度差异通常较低,并且活性甲烷菌和产甲烷菌在所有三个位置和深度共存,与CH_(4)和O_(2)的浓度或植物种类无关。

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