...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Genomic Diversity of Phages Infecting Probiotic Strains of Lactobacillus paracasei
【24h】

Genomic Diversity of Phages Infecting Probiotic Strains of Lactobacillus paracasei

机译：感染副干酪乳杆菌益生菌的噬菌体的基因组多样性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Strains of the Lactobacillus casei group have been extensively studied because some are used as probiotics in foods. Conversely, their phages have received much less attention. We analyzed the complete genome sequences of five L. paracasei temperate phages: C_(L)1, C_(L)2, i Lp 84, i Lp 1308, and iA2. Only phage iA2 could not replicate in an indicator strain. The genome lengths ranged from 34,155 bp (iA2) to 39,474 bp (C_(L)1). Phages iA2 and i Lp 1308 (34,176 bp) possess the smallest genomes reported, thus far, for phages of the L. casei group. The GC contents of the five phage genomes ranged from 44.8 to 45.6%. As observed with many other phages, their genomes were organized as follows: genes coding for DNA packaging, morphogenesis, lysis, lysogeny, and replication. Phages C_(L)1, C_(L)2, and i Lp 1308 are highly related to each other. Phage i Lp 84 was also related to these three phages, but the similarities were limited to gene products involved in DNA packaging and structural proteins. Genomic fragments of phages C_(L)1, C_(L)2, i Lp 1308, and i Lp 84 were found in several genomes of L. casei strains. Prophage iA2 is unrelated to these four phages, but almost all of its genome was found in at least four L. casei strains. Overall, these phages are distinct from previously characterized Lactobacillus phages. Our results highlight the diversity of L. casei phages and indicate frequent DNA exchanges between phages and their hosts.
机译：干酪乳杆菌菌株已被广泛研究，因为其中一些被用作食品中的益生菌。相反，它们的噬菌体受到的关注要少得多。我们分析了五个副干酪乳杆菌温带噬菌体的完整基因组序列：C_（L）1，C_（L）2，i Lp 84，i Lp 1308和iA2。仅噬菌体iA2不能在指示菌株中复制。基因组长度范围从34155 bp（iA2）到39474 bp（C_（L）1）。迄今为止，对于干酪乳杆菌组的噬菌体，噬菌体iA2和i Lp 1308（34,176 bp）拥有最小的基因组。五个噬菌体基因组的GC含量范围为44.8至45.6％。正如其他许多噬菌体所观察到的那样，它们的基因组组织如下：编码DNA包装，形态发生，裂解，溶原性和复制的基因。噬菌体C_（L）1，C_（L）2和i Lp 1308彼此高度相关。噬菌体I Lp 84也与这三个噬菌体有关，但相似性仅限于涉及DNA包装和结构蛋白的基​​因产物。在干酪乳杆菌菌株的几个基因组中发现了噬菌体C_（L）1，C_（L）2，i Lp 1308和i Lp 84的基因组片段。噬菌体iA2与这四个噬菌体无关，但几乎在至少四个干酪乳杆菌菌株中发现了其全部基因组。总体而言，这些噬菌体不同于先前表征的乳杆菌噬菌体。我们的结果突出了干酪乳杆菌噬菌体的多样性，并表明噬菌体与其宿主之间频繁的DNA交换。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号