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首页> 外文期刊>Applied Microbiology >Agrobacterium tumefaciens Zur Regulates the High-Affinity Zinc Uptake System TroCBA and the Putative Metal Chaperone YciC, along with ZinT and ZnuABC, for Survival under Zinc-Limiting Conditions
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Agrobacterium tumefaciens Zur Regulates the High-Affinity Zinc Uptake System TroCBA and the Putative Metal Chaperone YciC, along with ZinT and ZnuABC, for Survival under Zinc-Limiting Conditions

机译：根癌农杆菌Zur调节高亲和力的锌吸收系统TroCBA和推定的金属伴侣YciC，以及ZinT和ZnuABC，以在限锌条件下存活

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Agrobacterium tumefaciens has a cluster of genes (Atu3178, Atu3179, and Atu3180) encoding an ABC-type transporter, here named troA , troB , and troC , respectively, which is shown here to be a zinc-specific uptake system. Reverse transcription (RT)-PCR analysis confirmed that troA , troB , and troC are cotranscribed, with troC as the first gene of the operon. The yciC (Atu3181) gene is transcribed in the opposite orientation to that of the troCBA operon and belongs to a metal-binding GTPase family. Expression of troCBA and yciC was inducible under zinc-limiting conditions and was controlled by the zinc uptake regulator, Zur. Compared to the wild type, the mutant strain lacking troC was hypersensitive to a metal chelator, EDTA, and the phenotype could be rescued by the addition of zinc, while the strain with a single yciC mutation showed no phenotype. However, yciC was important for survival under zinc limitation when either troC or zinT was inactivated. The periplasmic zinc-binding protein, ZinT, could not function when TroC was inactivated, suggesting that ZinT may interact with TroCBA in zinc uptake. Unlike many other bacteria, the ABC-type transporter ZnuABC was not the major zinc uptake system in A. tumefaciens . However, the important role of A. tumefaciens ZnuABC was revealed when TroCBA was impaired. The strain containing double mutations in the znuA and troC genes exhibited a growth defect in minimal medium. A. tumefaciens requires cooperation of zinc uptake systems and zinc chaperones, including TroCBA, ZnuABC, ZinT, and YciC, for survival under a wide range of zinc-limiting conditions.IMPORTANCE Both host and pathogen battle over access to essential metals, including zinc. In low-zinc environments, physiological responses that make it possible to acquire enough zinc are important for bacterial survival and could determine the outcome of host-pathogen interactions. A. tumefaciens was found to operate a novel pathway for zinc uptake in which ZinT functions in concert with the high-affinity zinc importer TroCBA.
机译：根癌土壤杆菌具有编码ABC型转运蛋白的基因簇（Atu3178，Atu3179和Atu3180），在这里分别命名为troA，troB和troC，在此显示为锌特异性摄取系统。逆转录（RT）-PCR分析证实troA，troB和troC是共转录的，其中troC是操纵子的第一个基因。 yciC（Atu3181）基因以与troCBA操纵子相反的方向转录，并且属于金属结合GTPase家族。 troCBA和yciC的表达在锌限制条件下是可诱导的，并受锌摄取调节剂Zur的控制。与野生型相比，缺少troC的突变菌株对金属螯合剂EDTA敏感，通过添加锌可以挽救表型，而具有yciC突变的菌株没有表型。但是，当troC或zinT失活时，yciC对于在锌限制下的存活至关重要。 TroC失活时，周质锌结合蛋白ZinT无法起作用，这表明ZinT可能与TroCBA相互作用以吸收锌。与许多其他细菌不同，ABC型转运蛋白ZnuABC不是根癌土壤杆菌的主要锌吸收系统。但是，当TroCBA受损时，揭示了根癌农杆菌ZnuABC的重要作用。 znuA和troC基因中含有双突变的菌株在基本培养基中显示出生长缺陷。根癌农杆菌需要锌吸收系统和锌伴侣蛋白（包括TroCBA，ZnuABC，ZinT和YciC）的配合才能在各种锌限制条件下生存。重要提示宿主和病原体都在争夺获取基本金属（包括锌）的斗争。在低锌环境中，能够获取足够锌的生理反应对于细菌存活很重要，并可能决定宿主与病原体相互作用的结果。发现根癌农杆菌操作一种新的锌吸收途径，其中ZinT与高亲和力的锌进口商TroCBA协同起作用。

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