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首页> 外文期刊>Applied Microbiology >The Blue-Light Photoreceptor Sfwc-1 Gene Regulates the Phototropic Response and Fruiting-Body Development in the Homothallic Ascomycete Sordaria fimicola
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The Blue-Light Photoreceptor Sfwc-1 Gene Regulates the Phototropic Response and Fruiting-Body Development in the Homothallic Ascomycete Sordaria fimicola

机译：蓝光感光细胞Sfwc-1基因调节同型子囊拟南芥（Sordaria fimicola）的光致反应和子实体发育。

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Sordaria fimicola, a coprophilous ascomycete, is a homothallic fungus that can undergo sexual differentiation with cellular and morphological changes followed by multicellular tissue development to complete its sexual cycle. In this study, we identified and characterized the blue-light photoreceptor gene in S. fimicola. The S. fimicola white collar-1 photoreceptor (SfWC-1) contains light-oxygen-voltage-sensing (LOV), Per-Arnt-Sim (PAS), and other conserved domains and is homologous to the WC-1 blue-light photoreceptor of Neurospora crassa. The LOV domain of Sfwc-1 was deleted by homologous recombination using Agrobacterium-mediated protoplast transformation. The Sfwc-1(Δlov) mutant showed normal vegetative growth but produced less carotenoid pigment under illumination. The mutant showed delayed and less-pronounced fruiting-body formation, was defective in phototropism of the perithecial beaks, and lacked the fruiting-body zonation pattern compared with the wild type under the illumination condition. Gene expression analyses supported the light-induced functions of the Sfwc-1 gene in the physiology and developmental process of perithecial formation in S. fimicola. Moreover, green fluorescent protein (GFP)-tagged SfWC-1 fluorescence signals were transiently strong upon light induction and prominently located inside the nuclei of living hyphae. Our studies focused on the putative blue-light photoreceptor in a model ascomycete and contribute to a better understanding of the photoregulatory functions and networks mediated by the evolutionarily conserved blue-light photoreceptors across diverse fungal phyla.IMPORTANCESordaria sp. has been a model for study of fruiting-body differentiation in fungi. Several environmental factors, including light, affect cellular and morphological changes during multicellular tissue development. Here, we created a light-oxygen-voltage-sensing (LOV) domain-deleted Sfwc-1 mutant to study blue-light photoresponses in Sordaria fimicola. Phototropism and rhythmic zonation of perithecia were defective in the Sfwc-1(Δlov) mutant. Moreover, fruiting-body development in the mutant was reduced and also significantly delayed. Gene expression analysis and subcellular localization study further revealed the light-induced differential gene expression and cellular responses upon light stimulation in S. fimicola.
机译：Fiord Sordaria fimicola是一种同型真菌，它是一种同型真菌，可以通过细胞和形态变化发生性分化，然后发生多细胞组织发育以完成其性周期。在这项研究中，我们确定和表征了S. fimicola中的蓝光感光基因。 S. fimicola白领1感光体（SfWC-1）包含光氧电压感测（LOV），Per-Arnt-Sim（PAS）和其他保守域，并且与WC-1蓝光同源神经孢霉的光感受器。使用农杆菌介导的原生质体转化通过同源重组缺失Sfwc-1的LOV结构域。 Sfwc-1（Δlov）突变体显示正常的营养生长，但在光照下产生的类胡萝卜素色素较少。与光照条件下的野生型相比，该突变体显示出延迟的和少读出的子实体形成，在果皮喙的向光性上有缺陷，并且缺乏子实体分区模式。基因表达分析支持Sfwc-1基因的光诱导功能，在S. fimicola的皮膜形成的生理和发育过程中发挥作用。此外，绿色荧光蛋白（GFP）标记的SfWC-1荧光信号在光诱导时短暂强，并显着位于活菌丝的核内。我们的研究集中于子囊模型中的推定蓝光感受器，有助于更好地理解跨不同真菌门的进化上保守的蓝光感受器介导的光调节功能和网络。已经成为研究真菌子实体分化的模型。包括光在内的几种环境因素会影响多细胞组织发育过程中的细胞和形态变化。在这里，我们创建了一个删除光氧电压感测（LOV）域的Sfwc-1突变体，以研究在淡淡紫檀中的蓝光光响应。 Sfwc-1（Δlov）突变体的外周膜的向光性和节律性节律性缺陷。此外，突变体中子实体的发育减少并且也显着延迟。基因表达分析和亚细胞定位研究进一步揭示了光诱导的线虫中光诱导的差异基因表达和细胞反应。

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