Production of Aflatoxin on Wheat and Oats: Measurement with a Recording Densitometer

R. D. Stubblefield; O. L. Shotwell; C. W. Hesseltine; M. L. Smith; H. H. Hall

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Production of Aflatoxin on Wheat and Oats: Measurement with a Recording Densitometer

机译：小麦和燕麦上黄曲霉毒素的生产：记录密度计的测量

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A method has been developed for the production of aflatoxin by growing Aspergillus flavus NRRL 3145 on solid substrate wheat. Optimal yields of 900 μg of aflatoxin G1 and 900 μg of aflatoxin B1 per g of substrate were obtained in 4 to 5 days at 28 C. A study of aflatoxin production on hulls and groats of oats and on whole oats by A. flavus strains NRRL 2999, NRRL 3000, and NRRL 3145 revealed that aflatoxin was produced on all three substrates, although production was very slight on hulls. Strain NRRL 3145 grown on solid substrate groats produced the largest amounts of aflatoxin: 580 μg of B1 and 450 μg of G1 per g of substrate. A densitometric method for reading thin-layer chromatographic plates is described; this is more objective and more accurate than the visual methods previously used for the determination of all four aflatoxins.

机译：已经开发了通过在固体基质小麦上生长黄曲霉NRRL 3145来生产黄曲霉毒素的方法。在28°C下4至5天，每克底物可获得900μg黄曲霉毒素G1和900μg黄曲霉毒素B1的最佳产量。黄曲霉菌NRRL对燕麦的壳和碎粒以及整个燕麦上黄曲霉毒素产量的研究2999，NRRL 3000和NRRL 3145揭示了在所有三种底物上都产生了黄曲霉毒素，尽管在船体上产量很小。在固体基质碎粒上生长的菌株NRRL 3145产生了最大量的黄曲霉毒素：每g基质580μgB1和450μgG1。描述了读取薄层色谱板的光密度法;与以前用于测定所有四种黄曲霉毒素的视觉方法相比，这更客观，更准确。

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《Applied Microbiology》 |1967年第1期|共5页
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R. D. Stubblefield; O. L. Shotwell; C. W. Hesseltine; M. L. Smith; H. H. Hall;
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中图分类微生物学;
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6. Production of Aflatoxin on Wheat and Oats: Measurement with a Recording Densitometer [O] . R. D. Stubblefield, O. L. Shotwell, C. W. Hesseltine, 1967

机译：小麦和燕麦上黄曲霉毒素的生产：记录密度计的测量
7. Production of Aflatoxin on Wheat and Oats: Measurement with a Recording Densitometer [O] . R. D. Stubblefield, O. L. Shotwell, C. W. Hesseltine, 1967

机译：小麦和燕麦上黄曲霉毒素的生产：用记录密度计测量

Production of Aflatoxin on Wheat and Oats: Measurement with a Recording Densitometer

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