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首页> 外文期刊>Applied Microbiology >Influence of Soil Characteristics on the Diversity of Bacteria in the Southern Brazilian Atlantic Forest
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Influence of Soil Characteristics on the Diversity of Bacteria in the Southern Brazilian Atlantic Forest

机译：土壤特征对巴西南部大西洋森林细菌多样性的影响

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The Brazilian Atlantic Forest is one of the 25 biodiversity hot spots in the world. Although the diversity of its fauna and flora has been studied fairly well, little is known of its microbial communities. In this work, we analyzed the Atlantic Forest ecosystem to determine its bacterial biodiversity, using 16S rRNA gene sequencing, and correlated changes in deduced taxonomic profiles with the physicochemical characteristics of the soil. DNAs were purified from soil samples, and the 16S rRNA gene was amplified to construct libraries. Comparison of 754 independent 16S rRNA gene sequences from 10 soil samples collected along a transect in an altitude gradient showed the prevalence of Acidobacteria (63%), followed by Proteobacteria (25.2%), Gemmatimonadetes (1.6%), Actinobacteria (1.2%), Bacteroidetes (1%), Chloroflexi (0.66%), Nitrospira (0.4%), Planctomycetes (0.4%), Firmicutes (0.26%), and OP10 (0.13%). Forty-eight sequences (6.5%) represented unidentified bacteria. The Shannon diversity indices of the samples varied from 4.12 to 3.57, indicating that the soils have a high level of diversity. Statistical analysis showed that the bacterial diversity is influenced by factors such as altitude, Ca~(2+)/Mg~(2+) ratio, and Al~(3+) and phosphorus content, which also affected the diversity within the same lineage. In the samples analyzed, pH had no significant impact on diversity.
机译：巴西大西洋森林是世界上25个生物多样性热点之一。尽管已经对其动植物群的多样性进行了很好的研究，但对其微生物群落知之甚少。在这项工作中，我们使用16S rRNA基因测序分析了大西洋森林生态系统，以确定其细菌生物多样性，并将推导的生物分类特征的变化与土壤的理化特性相关联。从土壤样品中纯化DNA，并扩增16S rRNA基因以构建文库。在海拔梯度上对沿样线收集的10个土壤样品中的754个独立的16S rRNA基因序列进行比较，结果发现，嗜酸菌（63％），紧随其后的是变形杆菌（25.2％），芽孢杆菌（1.6％），放线菌（1.2％），拟杆菌（1％），柔韧性（0.66％），硝化螺菌（0.4％），扁平菌（0.4％），硬毛菌（0.26％）和OP10（0.13％）。四十八个序列（6.5％）代表未鉴定的细菌。样品的香农多样性指数在4.12至3.57之间变化，表明土壤具有较高的多样性。统计分析表明，细菌的多样性受海拔，Ca〜（2 +）/ Mg〜（2+）比，Al〜（3+）和磷含量等因素的影响，也影响同一谱系内的多样性。 。在分析的样品中，pH值对多样性没有明显影响。

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