...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Monitoring the Wet-Heat Inactivation Dynamics of Single Spores of Bacillus Species by Using Raman Tweezers, Differential Interference Contrast Microscopy, and Nucleic Acid Dye Fluorescence Microscopy
【24h】

Monitoring the Wet-Heat Inactivation Dynamics of Single Spores of Bacillus Species by Using Raman Tweezers, Differential Interference Contrast Microscopy, and Nucleic Acid Dye Fluorescence Microscopy

机译：通过使用拉曼镊子，微分干涉对比显微镜和核酸染料荧光显微镜监测芽孢杆菌物种的单个孢子的湿热失活动力学。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dynamic processes during wet-heat treatment of individual spores of Bacillus cereus , Bacillus megaterium , and Bacillus subtilis at 80 to 90°C were investigated using dual-trap Raman spectroscopy, differential interference contrast (DIC) microscopy, and nucleic acid stain (SYTO 16) fluorescence microscopy. During spore wet-heat treatment, while the spores' 1:1 chelate of Ca~(2+) with dipicolinic acid (CaDPA) was released rapidly at a highly variable time T _(lag), the levels of spore nucleic acids remained nearly unchanged, and the T _(lag) times for individual spores from the same preparation were increased somewhat as spore levels of CaDPA increased. The brightness of the spores' DIC image decreased by ～50% in parallel with CaDPA release, and there was no spore cortex hydrolysis observed. The lateral diameters of the spores' DIC image and SYTO 16 fluorescence image also decreased in parallel with CaDPA release. The SYTO 16 fluorescence intensity began to increase during wet-heat treatment at a time before T _(lag) and reached maximum at a time slightly later than T _(release). However, the fluorescence intensities of wet-heat-inactivated spores were ～15-fold lower than those of nutrient-germinated spores, and this low SYTO 16 fluorescence intensity may be due in part to the low permeability of the dormant spores' inner membranes to SYTO 16 and in part to nucleic acid denaturation during the wet-heat treatment.
机译：使用双阱拉曼光谱，微分干涉对比（DIC）显微镜和核酸染色（SYTO 16）对蜡状芽孢杆菌，巨大芽孢杆菌和枯草芽孢杆菌的单个孢子在80至90°C的湿热处理过程中的动态过程进行了研究。 ）荧光显微镜。在孢子湿热处理过程中，孢子与吡啶二羧酸（CaDPA）的Ca〜（2+）1：1螯​​合物在高度可变的时间T _（lag）迅速释放，但孢子核酸的水平保持接近孢子水平随着CaDPA孢子水平的增加而有所增加，并且相同制备物中单个孢子的T_（lag）时间有所增加。与CaDPA释放平行，孢子DIC图像的亮度降低了约50％，并且没有观察到孢子皮层水解。孢子的DIC图像和SYTO 16荧光图像的横向直径也随CaDPA释放而减小。 SYTO 16荧光强度在湿热处理期间在T_（滞后）之前开始增加，并在稍晚于T_（释放）的时间达到最大值。然而，湿热灭活的孢子的荧光强度比养分发芽的孢子的荧光强度低约15倍，而这种低的SYTO 16荧光强度可能部分是由于休眠孢子的内膜对SYTO 16，部分原因在于湿热处理中的核酸变性。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号